• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Modification of the Reichert FC-2 cryoattachment to improve ultrathin sectioning reproducibility.

作者信息

Leunissen J L, Pluygers H W, Elbers P F

出版信息

J Microsc. 1979 Dec;117(3):355-62. doi: 10.1111/j.1365-2818.1979.tb04692.x.

DOI:10.1111/j.1365-2818.1979.tb04692.x
PMID:522132
Abstract

Modifications of the Reichert FC-2 cryomicrotome attachment which facilitate sectioning of frozen material are described. Most valuable was the construction of a specimen holder chuck made of brass and connected to the specimen arm with a glass rod. Other changes include improved shielding from heat influx, a constant nitrogen pressure cooling system and a stable cryochamber mount. These modifications considerably improve reproducibility.

摘要

相似文献

1
Modification of the Reichert FC-2 cryoattachment to improve ultrathin sectioning reproducibility.
J Microsc. 1979 Dec;117(3):355-62. doi: 10.1111/j.1365-2818.1979.tb04692.x.
2
Preparation of cryosections with a modified Sorvall MT2B ultramicrotome and cryoattachment.
J Microsc. 1982 Apr;126(Pt 1):63-75. doi: 10.1111/j.1365-2818.1982.tb00357.x.
3
An ultrathin sectioning alignment tool with application to cell monolayers.一种应用于细胞单层的超薄切片对齐工具。
J Electron Microsc Tech. 1989 Feb;11(2):172-3. doi: 10.1002/jemt.1060110214.
4
Methods for improved light microscope microtomy.改进光学显微镜切片术的方法。
Stain Technol. 1979 Mar;54(2):53-69. doi: 10.3109/10520297909112636.
5
Ultramicrotomy for biological electron microscopy.用于生物电子显微镜的超薄切片术。
Methods Mol Biol. 2007;369:67-96. doi: 10.1007/978-1-59745-294-6_5.
6
A device for the reproducible spreading of cryoultramicrotome sections.
J Microsc. 1979 Jul;116(2):265-70. doi: 10.1111/j.1365-2818.1979.tb00207.x.
7
Preparation of fresh-frozen dried ultrathin sections by drying without vacuum.通过非真空干燥制备新鲜冷冻干燥超薄切片。
Acta Anat (Basel). 1976;96(2):248-58. doi: 10.1159/000144677.
8
Improved methods for making and using glass knives.制造和使用玻璃刀的改进方法。
Biotech Histochem. 1997 May;72(3):129-34.
9
Vitreous carbon: a new material for making microtome knives.
Stain Technol. 1983 May;58(3):143-51. doi: 10.3109/10520298309066775.
10
Stabilizing ultrathin cryo-sections by freeze-drying.
J Microsc. 1978 Jan;112(1):39-46. doi: 10.1111/j.1365-2818.1978.tb01152.x.

引用本文的文献

1
Immunocytochemistry of gonadotropic cells and identification of cell types in ultrathin cryosections of the pituitary of the rainbow trout, Salmo gairdneri.虹鳟鱼（Salmo gairdneri）垂体超薄冷冻切片中促性腺细胞的免疫细胞化学及细胞类型鉴定
Cell Tissue Res. 1982;226(1):177-94. doi: 10.1007/BF00217092.