Hagler Herbert K
Department of Pathology, University of Texas, Southwestern Medical Center, Dallas, TX, USA.
Methods Mol Biol. 2007;369:67-96. doi: 10.1007/978-1-59745-294-6_5.
This chapter describes the practical details involved in the successful sectioning of plastic-embedded specimens for electron microscopy. The focus will be on those parts of the process that are really key to the successful sectioning of any biological material, i.e., the proper shape and size of the specimen, sharp knives and how to make them, and the orientation of specimen and production of ultrathin sections onto water-filled boats attached to either glass or diamond knives. This chapter is designed to assist the beginner in microtomy in understanding more fully the terms and basic principles involved in producing ultrathin sections of most plastic-embedded biological specimens.
本章介绍了用于电子显微镜检查的塑料包埋标本成功切片所涉及的实际细节。重点将放在该过程中对于任何生物材料成功切片真正关键的那些部分，即标本的合适形状和尺寸、锋利的刀具以及如何制作刀具，以及标本的取向和将超薄切片制作到附着在玻璃刀或钻石刀上的充满水的载玻片上。本章旨在帮助切片初学者更全面地理解制作大多数塑料包埋生物标本超薄切片所涉及的术语和基本原理。
