Wang W, Cai Z, Du K
Department of Biology, Nanjing Normal University, Peoples' Republic of China.
Biotech Histochem. 1997 May;72(3):129-34.
We have observed over time that the right side of a glass knife is the optimal cutting edge for microtomy if the counterpiece (heel opposite the edge) is controlled within 1 mm. The right cutting edge has been considered the "saw toothed" side and has not been used for ultrathin sectioning. We have observed that the right cutting edge is sharper and more durable than the left. Light and scanning electron microscopy were used to observe the cutting edge, and transmission electron microscopy was used to examine semithin and ultrathin sections of animal and plant tissues cut by the right and left sides of the cutting edge. The results indicate that the cutting edge becomes sharper and more durable from left to right. Both the quality and efficiency of ultrathin sectioning is improved by using the right cutting edge.
随着时间的推移，我们观察到，如果切片刀的对应面（与刀刃相对的刀跟）控制在1毫米以内，玻璃刀的右侧是切片的最佳刀刃。右侧刀刃一直被认为是“锯齿状”的一侧，未用于超薄切片。我们观察到右侧刀刃比左侧更锋利、更耐用。使用光学显微镜和扫描电子显微镜观察刀刃，并使用透射电子显微镜检查用刀刃左右两侧切割的动植物组织的半薄和超薄切片。结果表明，刀刃从左到右变得更锋利、更耐用。使用右侧刀刃可提高超薄切片的质量和效率。
