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[猿猴SA7和人类6型腺病毒DNA中的GC碱基对分布]

[GC-pair distribution in the DNA of simian SA7 and human type 6 adenoviruses].

作者信息

Dubichev A G, Parfenov N N, Chaplygina N M, Kalinina T I, Naroditskiĭ B S

出版信息

Vopr Virusol. 1979 Nov-Dec(6):599-603.

PMID:524856
Abstract

The buoyant density values in cesium chloride of DNA fragments of simian adenovirus SA7 obtained with restricting endonucleases of R. EcoRI, R. BamHI, R. SalI and human adenovirus type 6 DNA fragments obtained with restricting endonucleases of R. EcoRI and R. BamHI were determined. On the basis of the buoyant density values for these fragments the content of GC pairs in them was calculated. Using the known position of the fragments in the physical map of these DNAs, the distribution of GC pairs along the examined DNA molecules was constructed.

摘要

测定了用R. EcoRI、R. BamHI、R. SalI限制性内切酶获得的猿猴腺病毒SA7 DNA片段以及用R. EcoRI和R. BamHI限制性内切酶获得的人腺病毒6型DNA片段在氯化铯中的浮力密度值。根据这些片段的浮力密度值计算了其中GC碱基对的含量。利用这些DNA物理图谱中片段的已知位置,构建了GC碱基对沿所检测DNA分子的分布情况。

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