[猿猴腺病毒基因组。II. 具有转化活性的病毒DNA特定片段的鉴定]
[Simian adenovirus genome. II. Identification of specific fragments of viral DNA possessing transforming activity].
作者信息
Gibadulin R A, Dejkema R, Denisova T S, van Ormondt H
出版信息
Mol Biol (Mosk). 1982 Mar-Apr;16(2):315-21.
The antigenic variant of simian adenovirus 7 (SA7) DNA was cleaved by restriction endonucleases EcoRI, XbaI, BamHI, SalI. The resulted digests of viral DNA were tested for transforming activity using the "calcium" technique. It was shown that BamHI. XbaI and SalI digests transformed primary baby rat kidney cells as well as native viral DNA. The transforming activity of separated BamHI and SalI fragments was tested also. The viral DNA fragments with transforming activity (BamHI-B and SalI-B) were localised on the left of the physical map of the viral genome. It was also shown that fragment-transformed cell lines were able to form colonies in 0.33% agarose medium.
猿猴腺病毒7（SA7）DNA的抗原变体被限制性内切酶EcoRI、XbaI、BamHI、SalI切割。使用“钙”技术测试病毒DNA的酶切消化产物的转化活性。结果表明，BamHI、XbaI和SalI的酶切消化产物与天然病毒DNA一样能转化原代新生大鼠肾细胞。还测试了分离的BamHI和SalI片段的转化活性。具有转化活性的病毒DNA片段（BamHI - B和SalI - B）定位在病毒基因组物理图谱的左侧。还表明，片段转化的细胞系能够在0.33%的琼脂糖培养基中形成集落。
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