[通过特异性Bgl II和Hind III限制性内切酶对猿猴腺病毒7型（SA7）DNA进行片段化]
[Simian adenovirus type 7 (SA7) DNA fragmentation by specific R.Bgl II and R.Hind III endonucleases].
作者信息
Zavizion B A, Gol'dberg E V, Naroditskiĭ B S, Zolotarskaia D E, Tikhonenko T I
出版信息
Vopr Virusol. 1979 Nov-Dec(6):595-9.
The effect of restricting R. BgIII and R. HindIII endonucleases on the genome of simian adenovirus type 7 (SA7) was studied. The R. BgIII endonuclease has 5 restriction sites on SA7 DNA and produces 6 fragments with molecular weights of 10.0 X 10(6), 5,3 X 10(6), 3.6 X 10(6), 1.8 X 10(6), 0.6 X 10(6), and 0.5 X 10(6) daltons. R. HindIII, having 3 restriction sites, hydrolyses SA7 DNA into 4 fragments with molecular weights of 7.6 X 10(6), 5.9 X 10(6), 5,2 X 10(6), 3,1 X 10(6) daltons. The R. BgIII and R. HindIII fragments are arranged in the SA7 genome in the following order: B-A-C-E-D-F and C-D-B-A.
研究了限制酶BgIII和HindIII对7型猿猴腺病毒（SA7）基因组的作用。BgIII限制酶在SA7 DNA上有5个限制位点，产生6个片段，分子量分别为10.0×10⁶、5.3×10⁶、3.6×10⁶、1.8×10⁶、0.6×10⁶和0.5×10⁶道尔顿。HindIII有3个限制位点，将SA7 DNA水解成4个片段，分子量分别为7.6×10⁶、5.9×10⁶、5.2×10⁶、3.1×10⁶道尔顿。BgIII和HindIII片段在SA7基因组中的排列顺序如下：B - A - C - E - D - F和C - D - B - A。
Zotero 插件