Levisohn R, Spiegelman S
Proc Natl Acad Sci U S A. 1969 Jul;63(3):805-11. doi: 10.1073/pnas.63.3.805.
Experiments are described which demonstrate that it is possible to isolate in vitro a variety of mutant RNA molecules which exhibit qualitatively distinguishable phenotypes. The results suggest that precellular evolution could have involved selective forces of previously unsuspected diversity and subtlety. Suitable adjustment of the selective conditions leads to the isolation of variants optimally designed to compete successfully with the original viral nucleic acid. One of the properties that can be built into the variants is resistance to the presence of inhibitory analogues of the normal riboside triphosphates. Potentially, such variants could be used as antiviral devices in conjunction with the more usual chemotherapeutic agents.
本文描述了一些实验，这些实验表明，有可能在体外分离出多种表现出定性可区分表型的突变RNA分子。结果表明，细胞前进化可能涉及到以前未被怀疑的多样性和微妙性的选择力。选择性条件的适当调整导致分离出经过优化设计以与原始病毒核酸成功竞争的变体。可以构建到变体中的一种特性是对正常核苷三磷酸抑制类似物的存在具有抗性。潜在地，这种变体可以与更常用的化学治疗剂一起用作抗病毒装置。
