使用复制RNA分子进行的进一步细胞外达尔文实验：在不同选择条件下分离出的多种变体。
Further extracellular Darwinian experiments with replicating RNA molecules: diverse variants isolated under different selective conditions.
作者信息
Levisohn R, Spiegelman S
出版信息
Proc Natl Acad Sci U S A. 1969 Jul;63(3):805-11. doi: 10.1073/pnas.63.3.805.
Abstract
Experiments are described which demonstrate that it is possible to isolate in vitro a variety of mutant RNA molecules which exhibit qualitatively distinguishable phenotypes. The results suggest that precellular evolution could have involved selective forces of previously unsuspected diversity and subtlety. Suitable adjustment of the selective conditions leads to the isolation of variants optimally designed to compete successfully with the original viral nucleic acid. One of the properties that can be built into the variants is resistance to the presence of inhibitory analogues of the normal riboside triphosphates. Potentially, such variants could be used as antiviral devices in conjunction with the more usual chemotherapeutic agents.
摘要
本文描述了一些实验，这些实验表明，有可能在体外分离出多种表现出定性可区分表型的突变RNA分子。结果表明，细胞前进化可能涉及到以前未被怀疑的多样性和微妙性的选择力。选择性条件的适当调整导致分离出经过优化设计以与原始病毒核酸成功竞争的变体。可以构建到变体中的一种特性是对正常核苷三磷酸抑制类似物的存在具有抗性。潜在地，这种变体可以与更常用的化学治疗剂一起用作抗病毒装置。
相似文献
1
Further extracellular Darwinian experiments with replicating RNA molecules: diverse variants isolated under different selective conditions.使用复制RNA分子进行的进一步细胞外达尔文实验：在不同选择条件下分离出的多种变体。
Proc Natl Acad Sci U S A. 1969 Jul;63(3):805-11. doi: 10.1073/pnas.63.3.805.
2
Towards an experimental analysis of molecular self-organization and precellular Darwinian evolution.迈向分子自组织和前细胞达尔文进化的实验分析。
Naturwissenschaften. 1979 May;66(5):228-43. doi: 10.1007/BF00571603.
3
A replicating RNA molecule suitable for a detailed analysis of extracellular evolution and replication.一种适用于细胞外进化与复制详细分析的复制性RNA分子。
Proc Natl Acad Sci U S A. 1972 Oct;69(10):3038-42. doi: 10.1073/pnas.69.10.3038.
4
Quantitative analysis of mutation and selection in self-replicating RNA.自我复制RNA中突变与选择的定量分析。
Adv Space Res. 1992;12(4):191-7. doi: 10.1016/0273-1177(92)90172-t.
5
Extremely high genetic diversity in a single tumor points to prevalence of non-Darwinian cell evolution.单个肿瘤中极高的遗传多样性表明非达尔文细胞进化普遍存在。
Proc Natl Acad Sci U S A. 2015 Nov 24;112(47):E6496-505. doi: 10.1073/pnas.1519556112. Epub 2015 Nov 11.
6
Frequent coinfection reduces RNA virus population genetic diversity.频繁的共感染降低了 RNA 病毒种群遗传多样性。
J Hered. 2013 Sep-Oct;104(5):704-12. doi: 10.1093/jhered/est038. Epub 2013 Jul 4.
7
Novel Synthesis and Phenotypic Analysis of Mutant Clouds for Hepatitis E Virus Genotype 1.戊型肝炎病毒1型突变云的新型合成与表型分析
J Virol. 2018 Jan 30;92(4). doi: 10.1128/JVI.01932-17. Print 2018 Feb 15.
8
Minority report: hidden memory genomes in HIV-1 quasispecies and possible clinical implications.少数群体报告：HIV-1准种中的隐藏记忆基因组及其可能的临床意义。
AIDS Rev. 2008 Apr-Jun;10(2):93-109.
9
Template activity of brome mosaic virus-RNA components with soluble brome mosaic virus RNA polymerase.雀麦花叶病毒RNA组分与可溶性雀麦花叶病毒RNA聚合酶的模板活性
Intervirology. 1973;1(3):201-9. doi: 10.1159/000148847.
10
Kinetics of rapid RNA evolution in vitro.
J Mol Evol. 1991 Oct;33(4):343-56. doi: 10.1007/BF02102865.
引用本文的文献
1
A general and biomedical perspective of viral quasispecies.病毒准种的综合与生物医学视角。
RNA. 2025 Feb 19;31(3):429-443. doi: 10.1261/rna.080280.124.
2
Synthetic directed evolution for targeted engineering of plant traits.用于植物性状靶向工程的合成定向进化
Front Plant Sci. 2024 Sep 2;15:1449579. doi: 10.3389/fpls.2024.1449579. eCollection 2024.
3
Viruses in astrobiology.天体生物学中的病毒。
Front Microbiol. 2022 Oct 26;13:1032918. doi: 10.3389/fmicb.2022.1032918. eCollection 2022.
4
In vivo, in vitro and in silico: an open space for the development of microbe-based applications of synthetic biology.体内、体外和计算机模拟：合成生物学中基于微生物的应用的开放空间。
Microb Biotechnol. 2022 Jan;15(1):42-64. doi: 10.1111/1751-7915.13937. Epub 2021 Sep 27.
5
Ecology and Evolution in the RNA World Dynamics and Stability of Prebiotic Replicator Systems.RNA世界中的生态与进化：前生物复制子系统的动力学与稳定性
Life (Basel). 2017 Nov 27;7(4):48. doi: 10.3390/life7040048.
6
From Prebiotics to Probiotics: The Evolution and Functions of tRNA Modifications.从益生元到益生菌：转运RNA修饰的演变与功能
Life (Basel). 2016 Mar 14;6(1):13. doi: 10.3390/life6010013.
7
Directed Evolution: Past, Present and Future.定向进化：过去、现在与未来
AIChE J. 2013 May;59(5):1432-1440. doi: 10.1002/aic.13995.
8
The promise and peril of continuous in vitro evolution.连续体外进化的前景与风险。
J Mol Evol. 2005 Aug;61(2):253-63. doi: 10.1007/s00239-004-0307-1. Epub 2005 Jun 27.
9
In vitro evolution of terminal protein-containing genomes.含末端蛋白基因组的体外进化
Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):2921-6. doi: 10.1073/pnas.94.7.2921.
10
Smoothness within ruggedness: the role of neutrality in adaptation.
Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):397-401. doi: 10.1073/pnas.93.1.397.
本文引用的文献
1
Electrophoretic separation of viral nucleic acids on polyacrylamide gels.病毒核酸在聚丙烯酰胺凝胶上的电泳分离。
J Mol Biol. 1967 Jun 28;26(3):373-87. doi: 10.1016/0022-2836(67)90310-5.
2
In vitro synthesis of an infectious mutant RNA with a normal RNA replicase.利用正常RNA复制酶进行感染性突变RNA的体外合成。
Science. 1966 Jul 1;153(3731):64-7. doi: 10.1126/science.153.3731.64.
3
A two-dimensional fractionation procedure for radioactive nucleotides.一种用于放射性核苷酸的二维分级分离程序。
J Mol Biol. 1965 Sep;13(2):373-98. doi: 10.1016/s0022-2836(65)80104-8.
4
The cloning of a self-replicating RNA molecule.一种自我复制RNA分子的克隆。
Proc Natl Acad Sci U S A. 1968 Jul;60(3):866-72. doi: 10.1073/pnas.60.3.866.
5
An extracellular Darwinian experiment with a self-duplicating nucleic acid molecule.一项针对自我复制核酸分子的细胞外达尔文实验。
Proc Natl Acad Sci U S A. 1967 Jul;58(1):217-24. doi: 10.1073/pnas.58.1.217.
6
The synthesis of infectious RNA with a replicase purified according to its size and density.用根据其大小和密度纯化的复制酶合成感染性RNA。
Proc Natl Acad Sci U S A. 1966 Jun;55(6):1608-15. doi: 10.1073/pnas.55.6.1608.
7
Specific template requirments of RNA replicases.RNA复制酶的特定模板要求。
Proc Natl Acad Sci U S A. 1965 Aug;54(2):579-87. doi: 10.1073/pnas.54.2.579.
8
The sequence at the 5' terminus of a self-replicating variant of viral Q-beta ribonucleic acid.病毒Q-β核糖核酸自我复制变体5'末端的序列。
Biochemistry. 1968 Oct;7(10):3744-53. doi: 10.1021/bi00850a056.
Zotero 插件