影响酵母his4酶复合物的无义突变。
Nonsense mutations affecting the his4 enzyme complex of yeast.
作者信息
Shaffer B, Rytka J, Fink G R
出版信息
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1198-205. doi: 10.1073/pnas.63.4.1198.
Abstract
The his4 region of yeast contains the information necessary for the catalysis of three steps in the histidine biosynthetic pathway. The three activities specified by this region remain physically associated during gel filtration and ultracentrifugation and after extensive purification. Nonsense mutations in the "operator distal" his4C region lower the molecular weight of the two proximal activities. The proximal activities can, therefore, function without the his4C portion of the protein(s). These observations are compatible with the following three possibilities. The his4 region codes for: (1) an aggregate of three proteins forming a multiprotein complex; (2) a single protein with three catalytic activities; and (3) a single protein which is cleaved to form three proteins which remain associated.
摘要
酵母的his4区域包含组氨酸生物合成途径中三步催化所需的信息。该区域所指定的三种活性在凝胶过滤、超速离心过程中以及经过大量纯化后仍保持物理关联。“操纵基因远端”的his4C区域中的无义突变降低了两个近端活性的分子量。因此，近端活性在没有蛋白质的his4C部分的情况下也能发挥作用。这些观察结果与以下三种可能性相符。his4区域编码：(1) 形成多蛋白复合物的三种蛋白质的聚集体；(2) 具有三种催化活性的单一蛋白质；(3) 一种被切割形成三种仍保持关联的蛋白质的单一蛋白质。
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