糖尿病与衰老：培养的人成纤维细胞种植效率降低
Diabetes mellitus and aging: diminished planting efficiency of cultured human fibroblasts.
作者信息
Goldstein S, Littlefield J W, Soeldner J S
出版信息
Proc Natl Acad Sci U S A. 1969 Sep;64(1):155-60. doi: 10.1073/pnas.64.1.155.
Abstract
This work concerns the effect of age and the diabetic gene(s) on the growth capacity of skin fibroblasts in culture. Cells from normal subjects and the progeny of conjugal diabetics have similar lifespans after multiple passages in mass culture. The combined lifespans in vitro are inversely proportional to the age of the donor. When individual cells are plated, more of those from normal subjects are able to form colonies. The difference in plating efficiency is apparent when first tested after 20 generations of growth, persists at 30 and 40 generations, but disappears after 50 generations. This cell culture system should be useful in studying the inheritance of the diabetic gene(s), the pathogenesis of the diabetic state, and the relationship between aging and diabetes, both of which decrease plating efficiency.
摘要
这项研究关注年龄和糖尿病基因对培养的皮肤成纤维细胞生长能力的影响。来自正常受试者的细胞以及夫妻均患糖尿病者的后代细胞，在大规模培养中多次传代后具有相似的寿命。体外的总寿命与供体年龄成反比。当接种单个细胞时，来自正常受试者的细胞更多能够形成集落。接种效率的差异在生长20代后首次检测时就很明显，在30代和40代时依然存在，但在50代后消失。这种细胞培养系统在研究糖尿病基因的遗传、糖尿病状态的发病机制以及衰老与糖尿病之间的关系方面应该会很有用，这两者都会降低接种效率。
相似文献
1
Diabetes mellitus and aging: diminished planting efficiency of cultured human fibroblasts.糖尿病与衰老：培养的人成纤维细胞种植效率降低
Proc Natl Acad Sci U S A. 1969 Sep;64(1):155-60. doi: 10.1073/pnas.64.1.155.
2
Diabetes mellitus and genetic prediabetes. Decreased replicative capacity of cultured skin fibroblasts.糖尿病和遗传性糖尿病前期。培养的皮肤成纤维细胞复制能力下降。
J Clin Invest. 1979 Mar;63(3):358-70. doi: 10.1172/JCI109311.
3
Chronologic and physiologic age affect replicative life-span of fibroblasts from diabetic, prediabetic, and normal donors.chronological和生理年龄影响来自糖尿病、糖尿病前期和正常供体的成纤维细胞的复制寿命。 （注：原文中chronologic拼写有误，正确拼写为chronological ）
Science. 1978 Feb 17;199(4330):781-2. doi: 10.1126/science.622567.
4
Insulin-dependent childhood diabetes. Normal viability of cultured fibroblasts.胰岛素依赖型儿童糖尿病。培养的成纤维细胞具有正常活力。
Diabetes. 1978 Mar;27(3):338-41. doi: 10.2337/diab.27.3.338.
5
Cellular and molecular biological studies on diabetes mellitus.糖尿病的细胞与分子生物学研究
Pathol Biol (Paris). 1984 Feb;32(2):99-106.
6
Lifespans of diabetic and non-diabetic fibroblasts in vitro. Results of replicate determinations.体外培养的糖尿病和非糖尿病成纤维细胞的寿命。重复测定结果。
Exp Cell Res. 1980 Oct;129(2):345-50. doi: 10.1016/0014-4827(80)90502-9.
7
Pathological implications of cell aging in vitro.体外细胞衰老的病理学意义
Fed Proc. 1975 Jan;34(1):56-63.
8
Seeding efficiency, plating efficiency, and population doublings of human skin fibroblastlike cells: results of replicate testing.人皮肤成纤维细胞样细胞的接种效率、铺板效率和群体倍增：重复测试结果
In Vitro. 1983 Jun;19(6):504-14. doi: 10.1007/BF02619598.
9
Abnormal glucose transport and GLUT1 cell-surface content in fibroblasts and skeletal muscle from NIDDM and obese subjects.非胰岛素依赖型糖尿病（NIDDM）患者及肥胖者成纤维细胞和骨骼肌中葡萄糖转运异常及葡萄糖转运蛋白1（GLUT1）细胞表面含量变化
Diabetologia. 1997 Apr;40(4):421-9. doi: 10.1007/s001250050696.
10
Analytical review: the pathogenesis of diabetes mellitus and its relationship to biological aging.分析性综述：糖尿病的发病机制及其与生物衰老的关系。
Humangenetik. 1971;12(2):83-100. doi: 10.1007/BF00291463.
引用本文的文献
1
Untargeted metabolomics analysis identifies creatine, myo-inositol, and lipid pathway modulation in a murine model of tendinopathy.非靶向代谢组学分析鉴定出肌腱病小鼠模型中肌酸、肌醇和脂质代谢途径的调节。
J Orthop Res. 2022 Apr;40(4):965-976. doi: 10.1002/jor.25112. Epub 2021 Jun 17.
2
Acceleration of β Cell Aging Determines Diabetes and Senolysis Improves Disease Outcomes.β 细胞衰老加速决定糖尿病发生，衰老细胞清除可改善疾病转归。
Cell Metab. 2019 Jul 2;30(1):129-142.e4. doi: 10.1016/j.cmet.2019.05.006. Epub 2019 May 30.
3
In vitro Therapeutic Effects of Low Level Laser at mRNA Level on the Release of Skin Growth Factors from Fibroblasts in Diabetic Mice.低强度激光在mRNA水平对糖尿病小鼠成纤维细胞皮肤生长因子释放的体外治疗作用
Avicenna J Med Biotechnol. 2014 Apr;6(2):113-8.
4
Relationship between donor age and the replicative lifespan of human cells in culture: a reevaluation.供体年龄与培养的人类细胞复制寿命之间的关系：重新评估
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10614-9. doi: 10.1073/pnas.95.18.10614.
5
Cellular aging, destabilization, and cancer.细胞衰老、不稳定与癌症。
Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1825-30. doi: 10.1073/pnas.93.5.1825.
6
Donor site, age, and health affect fibroblast growth in culture.供体部位、年龄和健康状况会影响培养中的成纤维细胞生长。
In Vitro Cell Dev Biol Anim. 1995 Jul-Aug;31(7):494-6. doi: 10.1007/BF02634025.
7
Tissue culture in aging research: present status and prospects.衰老研究中的组织培养：现状与展望
Experientia. 1981 Oct 15;37(10):1050-3. doi: 10.1007/BF02085004.
8
Wound healing in normal and diabetic Chinese hamsters.正常和糖尿病中国仓鼠的伤口愈合
Diabetologia. 1981 Oct;21(4):394-401. doi: 10.1007/BF00252688.
9
Decreased sensitivity of old and progeric human fibroblasts to a preparation of factors with insulinlike activity.老年和早衰人类成纤维细胞对具有胰岛素样活性的因子制剂的敏感性降低。
J Clin Invest. 1981 Oct;68(4):988-94. doi: 10.1172/jci110353.
10
Cell culture aging.细胞培养老化
Mol Cell Biochem. 1981 May 26;36(3):169-76. doi: 10.1007/BF02357034.
本文引用的文献
1
Xenon Hexafluoride: Preparation of Pure Form and Melting Point.六氟化氙：纯态制备和熔点。
Science. 1964 Aug 14;145(3633):701-2. doi: 10.1126/science.145.3633.701.
2
CARBOHYDRATE TOLERANCE AND DIABETES IN A TOTAL COMMUNITY, TECUMSEH, MICHIGAN. 1. EFFECTS OF AGE, SEX, AND TEST CONDITIONS ON ONE-HOUR GLUCOSE TOLERANCE IN ADULTS.密歇根州蒂科姆西整个社区的碳水化合物耐受性与糖尿病。1. 年龄、性别和测试条件对成年人一小时葡萄糖耐量的影响。
Diabetes. 1965 Jul;14:413-23. doi: 10.2337/diab.14.7.413.
3
THE LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS.人二倍体细胞株的体外寿命有限。
Exp Cell Res. 1965 Mar;37:614-36. doi: 10.1016/0014-4827(65)90211-9.
4
MULTIFACTORIAL INHERITANCE: A POSSIBLE HYPOTHESIS FOR DIABETES.多因素遗传：糖尿病的一种可能假说。
Diabetes. 1964 Sep-Oct;13:462-71. doi: 10.2337/diab.13.5.462.
5
CLONAL GROWTH OF PRIMARY HUMAN CELL CULTURES.原代人细胞培养的克隆生长
Exp Cell Res. 1964 Feb;33:495-507. doi: 10.1016/0014-4827(64)90014-x.
6
Culture characteristics of human fibroblasts propagated serially.人成纤维细胞连续传代培养的特性
Am J Hyg. 1957 Sep;66(2):235-43. doi: 10.1093/oxfordjournals.aje.a119897.
7
Alkaline phosphatase "constitutive" clones: evidence for de-novo heterogeneity of established human skin fibroblast strains.碱性磷酸酶“组成型”克隆：已建立的人皮肤成纤维细胞系存在从头异质性的证据。
Exp Cell Res. 1967 Jan;45(1):72-84. doi: 10.1016/0014-4827(67)90113-9.
8
The division cycle and RNA-synthesis in diploid human cells at different passage levels in vitro.体外不同传代水平的二倍体人类细胞中的分裂周期与RNA合成
Exp Cell Res. 1966 Jun;42(3):673-84. doi: 10.1016/0014-4827(66)90280-1.
9
Effect of insulin on the conversion of glucose-C-14 to C-14-O2 by normal and diabetic fibroblasts in culture.胰岛素对培养的正常及糖尿病成纤维细胞将葡萄糖 - C - 14 转化为 C - 14 - O₂ 的影响。
Diabetes. 1969 Aug;18(8):545-9. doi: 10.2337/diab.18.8.545.
10
Diabetes mellitus in twins.双胞胎中的糖尿病
Diabetes. 1968 Nov;17(11):693-704. doi: 10.2337/diab.17.11.693.
Zotero 插件