Goldstein S, Littlefield J W, Soeldner J S
Proc Natl Acad Sci U S A. 1969 Sep;64(1):155-60. doi: 10.1073/pnas.64.1.155.
This work concerns the effect of age and the diabetic gene(s) on the growth capacity of skin fibroblasts in culture. Cells from normal subjects and the progeny of conjugal diabetics have similar lifespans after multiple passages in mass culture. The combined lifespans in vitro are inversely proportional to the age of the donor. When individual cells are plated, more of those from normal subjects are able to form colonies. The difference in plating efficiency is apparent when first tested after 20 generations of growth, persists at 30 and 40 generations, but disappears after 50 generations. This cell culture system should be useful in studying the inheritance of the diabetic gene(s), the pathogenesis of the diabetic state, and the relationship between aging and diabetes, both of which decrease plating efficiency.
这项研究关注年龄和糖尿病基因对培养的皮肤成纤维细胞生长能力的影响。来自正常受试者的细胞以及夫妻均患糖尿病者的后代细胞，在大规模培养中多次传代后具有相似的寿命。体外的总寿命与供体年龄成反比。当接种单个细胞时，来自正常受试者的细胞更多能够形成集落。接种效率的差异在生长20代后首次检测时就很明显，在30代和40代时依然存在，但在50代后消失。这种细胞培养系统在研究糖尿病基因的遗传、糖尿病状态的发病机制以及衰老与糖尿病之间的关系方面应该会很有用，这两者都会降低接种效率。
