一种针对噬菌体T4信使核糖核酸的基因检测方法。
A genetic assay for mRNA's of phage T4.
作者信息
Jayaraman R, Goldberg E B
出版信息
Proc Natl Acad Sci U S A. 1969 Sep;64(1):198-204. doi: 10.1073/pnas.64.1.198.
Abstract
A method has been developed by which many gene-specific mRNA's in T4-infected cells can be quantitatively assayed. The method involves separation of complementary strands of phage T4 DNA, hybridization of the strands with RNA, digestion of nonhybridized regions of DNA with an endonuclease specific for single-stranded DNA, and assay of protected genetic markers by transformation. It has been shown that the gene gammaIIB is transcribed early from the light strand and that the gene 21 is transcribed late from the heavy strand.
摘要
已经开发出一种方法，通过该方法可以对T4感染细胞中的许多基因特异性mRNA进行定量测定。该方法包括分离噬菌体T4 DNA的互补链，使这些链与RNA杂交，用对单链DNA具有特异性的核酸内切酶消化DNA的非杂交区域，并通过转化测定受保护的遗传标记。结果表明，γIIB基因从轻链早期转录，而21基因从重链晚期转录。
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