Sherr C J, Uhr J W
Proc Natl Acad Sci U S A. 1969 Sep;64(1):381-7. doi: 10.1073/pnas.64.1.381.
Mouse myeloma cells were pulse-labeled in vitro with (3)H-D-glucosamine and (14)C-L-leucine. Analysis on sucrose gradients revealed incorporation of both isotopes into polyribosomes and release of most of such radioactivity after treatment of labeled cells in vitro with puromycin. A mixing experiment excluded the in vitro binding to unlabeled polyribosomes of (3)H-glucosamine in labeled post-ribosomal material. Polyribosomes labeled with (3)H-glucosamine were precipitated with antiserum specific for mouse immunoglobulin. By chromatography and gel filtration, the precipitable radioactivity was shown to be glucosamine covalently bound to heavy and light chains.
用（3）H-D-葡萄糖胺和（14）C-L-亮氨酸对小鼠骨髓瘤细胞进行体外脉冲标记。蔗糖梯度分析显示两种同位素都掺入多核糖体中，并且在用嘌呤霉素体外处理标记细胞后，大部分这种放射性物质被释放。混合实验排除了标记的核糖体后物质中（3）H-葡萄糖胺与未标记多核糖体的体外结合。用对小鼠免疫球蛋白特异的抗血清沉淀用（3）H-葡萄糖胺标记的多核糖体。通过色谱法和凝胶过滤，显示可沉淀的放射性是共价结合到重链和轻链上的葡萄糖胺。
