Freed J J, Mezger-Freed L
Proc Natl Acad Sci U S A. 1970 Feb;65(2):337-44. doi: 10.1073/pnas.65.2.337.
Two haploid cell lines have been established from androgenetic embryos of the frog, Rana pipiens; one line has been maintained in culture for 150 generations, the other for 200 generations. Karyotypes of the two lines agree well with the standard for the species although some chromosomes show small differences in length. The cells multiply in the same defined basal medium used for culture of other anuran cell lines; this medium consists of the usual amino acids, vitamins, and serum macromolecules plus an exogenous purine source. Both the haploids resemble "permanent" cell lines in their prolonged multiplication in culture. The two lines differ in their mode of growth, one being epithelial-like, the other forming an overlapping meshwork of fibroblast-like cells. Both have the low plating efficiency characteristic of "unaltered" cells. These two lines are exceptional in their ability to compete successfully with the diploid variants which arise by endomitosis or cell fusion and which usually overgrow the haploid population. The more vigorous line, RPH 68.2A, should provide the long-desired haploid material for genetic studies in cell culture.
已从牛蛙（Rana pipiens）的雄核发育胚胎中建立了两个单倍体细胞系；一个细胞系已在培养中传代150次，另一个传代200次。尽管一些染色体在长度上存在微小差异，但这两个细胞系的核型与该物种的标准核型非常吻合。这些细胞在用于培养其他无尾两栖类细胞系的相同限定基础培养基中增殖；该培养基由常见的氨基酸、维生素、血清大分子以及外源嘌呤源组成。这两个单倍体细胞系在培养中长时间增殖，类似于“永久性”细胞系。这两个细胞系的生长方式不同，一个类似上皮细胞，另一个形成成纤维细胞样细胞的重叠网络。两者都具有“未改变”细胞的低接种效率特征。这两个细胞系在与通过核内有丝分裂或细胞融合产生的二倍体变体成功竞争的能力方面表现出色，这些二倍体变体通常会过度生长单倍体群体。更具活力的细胞系RPH 68.2A应为细胞培养中的遗传研究提供长期以来所期望的单倍体材料。
