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溴化乙锭 - DNA复合物荧光发射各向异性的衰减。DNA内部运动的证据。

Decay of fluorescence emission anisotropy of the ethidium bromide-DNA complex. Evidence for an internal motion in DNA.

作者信息

Wahl P, Paoletti J, Le Pecq J B

出版信息

Proc Natl Acad Sci U S A. 1970 Feb;65(2):417-21. doi: 10.1073/pnas.65.2.417.

DOI:10.1073/pnas.65.2.417
PMID:5263774
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC282919/
Abstract

Evidence for an internal oscillatory Brownian motion in the DNA helix is obtained from the measurement of the decay of the fluorescence emission anisotropy of the ethidium bromide-DNA complex. The amplitude of the oscillation is found to be equal to 35 degrees and the relaxation time equal to 28 nanoseconds.

摘要

通过测量溴化乙锭 - DNA 复合物荧光发射各向异性的衰减,获得了 DNA 螺旋内部存在振荡布朗运动的证据。发现振荡幅度等于 35 度,弛豫时间等于 28 纳秒。

相似文献

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Decay of fluorescence emission anisotropy of the ethidium bromide-DNA complex. Evidence for an internal motion in DNA.溴化乙锭 - DNA复合物荧光发射各向异性的衰减。DNA内部运动的证据。
Proc Natl Acad Sci U S A. 1970 Feb;65(2):417-21. doi: 10.1073/pnas.65.2.417.
2
[Energy transfer in the DNA-ethidium bromide complex by means of the decay of fluorescence anisotropy].[通过荧光各向异性衰减研究DNA-溴化乙锭复合物中的能量转移]
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Fluorescence decay time characteristics of the complex between ethidium bromide and nucleic acids.溴化乙锭与核酸复合物的荧光衰减时间特性。
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The fluorescence anisotropy decay due to energy transfers occuring in the ethidium bromide-DNA complex. Determination of the deformation angle of the DNA helix.溴化乙锭 - DNA复合物中发生的能量转移导致的荧光各向异性衰减。DNA螺旋变形角的测定。
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本文引用的文献

1
A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.溴化乙锭与核酸之间的荧光复合物。物理化学特性
J Mol Biol. 1967 Jul 14;27(1):87-106. doi: 10.1016/0022-2836(67)90353-1.
2
Flow dichroism of DNA: a new apparatus and further studies.DNA的流动二色性:一种新装置及进一步研究
Biopolymers. 1969;7(3):335-52. doi: 10.1002/bip.1969.360070306.
3
Fluorescence spectroscopy of proteins.蛋白质的荧光光谱学
Science. 1968 Nov 1;162(3853):526-33. doi: 10.1126/science.162.3853.526.
4
Relaxation studies of the proflavine-DNA complex: the kinetics of an intercalation reaction.原黄素-DNA复合物的弛豫研究:嵌入反应的动力学
J Mol Biol. 1969 Feb 14;39(3):461-77. doi: 10.1016/0022-2836(69)90138-7.
5
[Study of the polarized fluorescence decay of rabbit gamma globulin conjugated with fluroescein isothiocyanate].[异硫氰酸荧光素标记兔γ球蛋白的偏振荧光衰减研究]
Biochim Biophys Acta. 1969 Jul 1;181(2):373-80.