Hibbert H R, Spencer R
J Hyg (Lond). 1970 Mar;68(1):131-5. doi: 10.1017/s0022172400028588.
The effect of various concentrations of sodium thioglycollate (0-0.05%) on the recovery of spores of four strains of clostridia was investigated. The results showed that thioglycollate at a concentration of 0.01% can be inhibitory to some clostridia. However, the results also indicated that the inhibitory effect is dependent upon the composition of the growth medium. The presence of glucose appeared to be particularly important in reducing the inhibitory effect. These findings support the view that the use of sodium thioglycollate in sterility test media should be discontinued.
研究了不同浓度的巯基乙酸钠(0 - 0.05%)对四种梭菌菌株孢子复苏的影响。结果表明,浓度为0.01%的巯基乙酸钠对某些梭菌有抑制作用。然而,结果也表明这种抑制作用取决于生长培养基的组成。葡萄糖的存在似乎对降低抑制作用尤为重要。这些发现支持了应停止在无菌试验培养基中使用巯基乙酸钠的观点。