Merad S, Boughenou D
Arch Inst Pasteur Alger. 1989;57:277-84.
We have used spore isolation by the sodium thioglycolate-lysozyme technique on collected stools. Of the 51 stools studied, we found 41% of Clostridium perfringens with an average ratio of 10(4) germs/gr. 15 strains were typical double hemolysis and trehalose positive-5 presented only one hemolysis and were trehalose negative. We only found a single strain of Clostridium difficile with a rate of 10(4) germs/gr in the stools of a 10 months infant.
我们采用硫代乙醇酸钠-溶菌酶技术对收集的粪便进行芽孢分离。在所研究的51份粪便样本中，我们发现41%的样本含有产气荚膜梭菌，平均每克粪便中有10⁴个菌。15株菌呈现典型的双溶血且海藻糖阳性，5株菌仅呈现一种溶血现象且海藻糖阴性。我们仅在一名10个月大婴儿的粪便中发现了一株艰难梭菌，每克粪便中的菌含量为10⁴个。
