Pellis N R, Kahan B D
J Immunol. 1975 Dec;115(6):1717-22.
Immunogenic materials were solubilized from two methylcholanthrene-induced fibrosarcomas using 3 M KCl and were assessed for antigen activity in an immunoprotection assay. Mice which had been pretreated with a single injection of extract (0.1 to 2.0 mg protein) were challenged 10 days later with 10(3), 10(4), or 10(5) tumor cells. Optimal protection, as evidenced by survival, tumor incidence, and prolonged latent period before neoplastic outgrowth, was found after pretreatment with 0.5 mg of crude KCl extract and challenge with 10(4) tumor cells. These results further reinforce the weak nature of tumor-specific antigens as immunogens and show that only in very restricted circumstances can solubilized preparations administered without adjuvant induce immunoprotection in syngeneic hosts.
使用3M氯化钾从两个甲基胆蒽诱导的纤维肉瘤中溶解免疫原性物质，并在免疫保护试验中评估其抗原活性。预先单次注射提取物（0.1至2.0毫克蛋白质）的小鼠在10天后接受10³、10⁴或10⁵个肿瘤细胞的攻击。在用0.5毫克粗氯化钾提取物预处理并接受10⁴个肿瘤细胞攻击后，观察到了最佳保护效果，这体现在生存率、肿瘤发生率以及肿瘤生长前潜伏期延长等方面。这些结果进一步证实了肿瘤特异性抗原作为免疫原的微弱性质，并表明只有在非常有限的情况下，无佐剂给药的溶解制剂才能在同基因宿主中诱导免疫保护。
