Mishra N C, Tatum E L
Proc Natl Acad Sci U S A. 1970 Jul;66(3):638-45. doi: 10.1073/pnas.66.3.638.
Biochemical and genetic evidence is presented that the rg-1 and rg-2 genes control the structure of the two isozymes of phosphoglucomutase (Phosphoglucomutase I and II) in Neurospora silophila. Results of kinetic and gel filtration studies show that the two phosphoglucomutases may exist in vitro either as separate or as a single but complex molecular species depending on the ionic concentration. The complex phosphoglucomutase molecule is suggested to be the physiologically active form of the enzyme. The change in the enzyme structure is discussed in relation to the morphological change in the ragged mutant strains.
有生化和遗传学证据表明，rg-1和rg-2基因控制嗜神经孢子菌中磷酸葡萄糖变位酶的两种同工酶（磷酸葡萄糖变位酶I和II）的结构。动力学和凝胶过滤研究结果表明，根据离子浓度，两种磷酸葡萄糖变位酶在体外可能以单独的形式存在，也可能以单一但复杂的分子形式存在。复合磷酸葡萄糖变位酶分子被认为是该酶的生理活性形式。本文讨论了酶结构的变化与粗糙突变菌株形态变化的关系。
