利用嵌合体对果蝇神经系统进行遗传学剖析。
Genetic dissection of the Drosophila nervous system by means of mosaics.
作者信息
Hotta Y, Benzer S
出版信息
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1156-63. doi: 10.1073/pnas.67.3.1156.
Abstract
Given a mutant having abnormal behavior, the anatomical domain responsible for the deficit may be identified by the use of genetic mosaicism. Individuals may be produced in which a portion of the body is mutant male while the rest is normal female. In such sex mosaics, or gynandromorphs, the division line between normal and mutant parts can occur in various orientations. Mutants of five different genes (cistrons) on the X-chromosome of Drosophila melanogaster, having various abnormalities in visual function, have been tested by this method. All of these have been found to be autonomous, i.e., a mutant eye always functions abnormally, regardless of the amount of normal tissue present elsewhere, indicating that the primary causes of the behavioral deficits in these mutants are within the eye.
摘要
对于具有异常行为的突变体，可通过基因嵌合体来确定导致缺陷的解剖学区域。可以培育出这样的个体，其身体的一部分是突变雄性，而其余部分是正常雌性。在这种性嵌合体或雌雄嵌合体中，正常部分和突变部分之间的分界线可以有各种走向。已经用这种方法对黑腹果蝇X染色体上五个不同基因（顺反子）的突变体进行了测试，这些突变体在视觉功能上有各种异常。所有这些都被发现是自主性的，即突变眼总是功能异常，无论其他地方存在多少正常组织，这表明这些突变体行为缺陷的主要原因在眼睛内部。
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