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人-小鼠体细胞杂种中两个人类X连锁基因的有丝分裂分离

Mitotic separation of two human X-linked genes in man--mouse somatic cell hybrids.

作者信息

Miller O J, Cook P R, Meera Khan P, Shin S, Siniscalco M

出版信息

Proc Natl Acad Sci U S A. 1971 Jan;68(1):116-20. doi: 10.1073/pnas.68.1.116.

DOI:10.1073/pnas.68.1.116
PMID:5276281
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC391174/
Abstract

SIX INTERSPECIFIC SOMATIC HYBRID CELL LINES WERE DERIVED FROM A MOUSE LINE DEFICIENT IN HYPOXANTHINE

guanine phosphoribosyltransferase (HGPRT) and human diploid cells with normal enzyme activity. Human HGPRT was present in all six hybrids and the clones derived from them. However, in two of the six, and in some clones from another two, human glucose-6-phosphate dehydrogenase (G6PD) was absent. Since the structural loci for both these enzymes are X-linked in man, these findings suggest that these two loci have separated quite frequently through chromosome breakage and that they must be rather far apart on the X chromosome.

摘要

六个种间体细胞杂交细胞系源自一个次黄嘌呤

鸟嘌呤磷酸核糖转移酶（HGPRT）缺陷的小鼠品系和具有正常酶活性的人二倍体细胞。人HGPRT存在于所有六个杂种细胞系及其衍生的克隆中。然而，在六个中的两个以及另外两个的一些克隆中，人葡萄糖-6-磷酸脱氢酶（G6PD）缺失。由于这两种酶的结构基因座在人类中都是X连锁的，这些发现表明这两个基因座通过染色体断裂相当频繁地分离，并且它们在X染色体上一定相距很远。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fc9/391174/5912ab3279ea/pnas00076-0125-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fc9/391174/c0c86071e65d/pnas00076-0125-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fc9/391174/708f59f55710/pnas00076-0125-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fc9/391174/5912ab3279ea/pnas00076-0125-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fc9/391174/c0c86071e65d/pnas00076-0125-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fc9/391174/708f59f55710/pnas00076-0125-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fc9/391174/5912ab3279ea/pnas00076-0125-c.jpg

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引用本文的文献

1
Results and prospects of chromosomal gene transfer between cultured mammalian cells.培养的哺乳动物细胞间染色体基因转移的结果与前景
Theor Appl Genet. 1978 May;52(3):97-104. doi: 10.1007/BF00264741.
2
The scattered distribution of actin genes in the mouse and human genomes.肌动蛋白基因在小鼠和人类基因组中的分散分布。
EMBO J. 1982;1(5):579-83. doi: 10.1002/j.1460-2075.1982.tb01211.x.
3
Thymidine kinase activity and human chromosome No. 18.
Biochem Genet. 1971 Oct;5(5):451-6. doi: 10.1007/BF00487134.

本文引用的文献

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Erythrocyte glucose-6-phosphate dehydrogenase deficiency: evidence of differences between Negroes and Caucasians with respect to this genetically determined trait.红细胞葡萄糖-6-磷酸脱氢酶缺乏症：关于这一由基因决定的性状，黑人和白种人之间存在差异的证据。
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HYBRID CELLS DERIVED FROM MOUSE AND MAN: ARTIFICIAL HETEROKARYONS OF MAMMALIAN CELLS FROM DIFFERENT SPECIES.源自小鼠和人类的杂交细胞：不同物种哺乳动物细胞的人工异核体
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Proc Natl Acad Sci U S A. 1971 Jul;68(7):1425-7. doi: 10.1073/pnas.68.7.1425.
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X-linkage of human phosphoglycerate kinase confirmed in man-mouse and man-Chinese hamster somatic cell hybrids.人类磷酸甘油酸激酶的X连锁在人-小鼠和人-中国仓鼠体细胞杂种中得到证实。
Am J Hum Genet. 1971 Nov;23(6):614-23.
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Linkage relationships of X-linked enzymes glucose-6-phosphate dehydrogenase and hypoxanthine guanine phosphoribosyltransferase: recombination in female offspring of compound heterozygotes.X连锁酶葡萄糖-6-磷酸脱氢酶和次黄嘌呤鸟嘌呤磷酸核糖基转移酶的连锁关系：复合杂合子雌性后代中的重组
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Absence of demonstrable linkage of human genes for enzymes of the purine and pyrimidine salvage pathways in human-mouse somatic cell hybrids.嘌呤和嘧啶补救途径中人类酶的基因在人-小鼠体细胞杂种中无明显连锁。
Biochem Genet. 1973 Jul;9(3):283-97. doi: 10.1007/BF00485741.
8
Restoration of hypoxanthine phosphoribosyl transferase activity in mouse 1R cells after fusion with chick-embryo fibroblasts.与鸡胚成纤维细胞融合后小鼠1R细胞中次黄嘌呤磷酸核糖基转移酶活性的恢复。
Proc Natl Acad Sci U S A. 1973 Jul;70(7):1998-2002. doi: 10.1073/pnas.70.7.1998.
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Genetics of human-mouse somatic cell hybrids: linkage of human genes for lactate dehydrogenase-A and esterase-A 4 .人-小鼠体细胞杂种的遗传学：人类乳酸脱氢酶-A和酯酶-A 4基因的连锁
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10
Cytological mapping of human X-linked genes by use of somatic cell hybrids involving an X-autosome translocation (mouse-hamster-human X-linked markers).利用涉及X-常染色体易位的体细胞杂种（小鼠-仓鼠-人类X连锁标记）对人类X连锁基因进行细胞遗传学定位。
Proc Natl Acad Sci U S A. 1972 Jan;69(1):69-73. doi: 10.1073/pnas.69.1.69.
小鼠成纤维细胞中鸟苷酸 - 次黄苷酸焦磷酸化酶缺乏的三个程度
Nature. 1964 Sep 12;203:1142-4. doi: 10.1038/2031142a0.
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DEMONSTRATION OF TWO POPULATIONS OF CELLS IN THE HUMAN FEMALE HETEROZYGOUS FOR GLUCOSE-6-PHOSPHATE DEHYDROGENASE VARIANTS.葡萄糖-6-磷酸脱氢酶变异体杂合子的人类女性中两种细胞群体的证明。
Proc Natl Acad Sci U S A. 1963 Sep;50(3):481-5. doi: 10.1073/pnas.50.3.481.
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Genetics of human cell line. IV. DNA-mediated heritable transformation of a biochemical trait.人类细胞系的遗传学。IV. 生物化学性状的DNA介导的可遗传转化
Proc Natl Acad Sci U S A. 1962 Dec 15;48(12):2026-34. doi: 10.1073/pnas.48.12.2026.
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Isolation and properties of hybrids between somatic mouse and Chinese hamster cells.小鼠体细胞与中国仓鼠细胞杂交种的分离及特性
Genetics. 1967 Sep;57(1):107-24. doi: 10.1093/genetics/57.1.107.
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Enzyme defect associated with a sex-linked human neurological disorder and excessive purine synthesis.与一种X连锁人类神经疾病及嘌呤过度合成相关的酶缺陷。
Science. 1967 Mar 31;155(3770):1682-4. doi: 10.1126/science.155.3770.1682.
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Properties and uses of human-mouse hybrid cell lines.人鼠杂交细胞系的特性与用途。
Nature. 1968 Dec 21;220(5173):1199-202. doi: 10.1038/2201199a0.
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Human-mouse somatic cell hybrids with single human chromosome (group E): link with thymidine kinase activity.携带单条人类染色体（E组）的人-鼠体细胞杂种：与胸苷激酶活性的关联
Science. 1968 Nov 29;162(3857):1005-6. doi: 10.1126/science.162.3857.1005.
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The nature of thymidine kinase in the human-mouse hybrid cell.人-鼠杂交细胞中胸苷激酶的性质。
Biochem Genet. 1969 Dec;3(6):583-90. doi: 10.1007/BF00485479.