Soriano P, Szabo P, Bernardi G
EMBO J. 1982;1(5):579-83. doi: 10.1002/j.1460-2075.1982.tb01211.x.
A hamster actin cDNA probe was used to localize actin genes on the major components of mouse and human DNAs, namely on the four families of fragments forming the bulk of these genomes. Over 20 EcoRI fragments hybridizing the probe could be detected; a different subset of these fragments was found in each component. Since the fragment families forming the major components of the mouse and human DNAs derive from very long chromosomal segments, the isochores , the presence of actin genes on all components provides evidence for their dispersion in both genomes. In situ hybridization of 125I-labeled probe to metaphase chromosomes in the presence of dextran sulfate confirmed this dispersion by showing that the 29-30 actin gene sites so identified are distributed on almost all chromosomes. Moreover, some human actin genes could be mapped on specific chromosomal segments; in particular, one gene was localized on the long arm of the X chromosome. Finally, three different mouse actin genes were isolated from a recombinant DNA library and previously investigated interspersed repeated sequences were identified in the vicinity of these genes.
使用仓鼠肌动蛋白cDNA探针将肌动蛋白基因定位在小鼠和人类DNA的主要成分上，即定位在构成这些基因组大部分的四类片段上。可以检测到20多个与该探针杂交的EcoRI片段；在每个成分中发现了这些片段的不同子集。由于构成小鼠和人类DNA主要成分的片段家族源自非常长的染色体区段，即等密度区带，所有成分上肌动蛋白基因的存在为其在两个基因组中的分散提供了证据。在硫酸葡聚糖存在的情况下，用125I标记的探针与中期染色体进行原位杂交，通过显示如此鉴定出的29 - 30个肌动蛋白基因位点分布在几乎所有染色体上，证实了这种分散。此外，一些人类肌动蛋白基因可以定位在特定的染色体区段上；特别是，一个基因定位在X染色体的长臂上。最后，从一个重组DNA文库中分离出三个不同的小鼠肌动蛋白基因，并在这些基因附近鉴定出先前研究过的散布重复序列。
