• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

与鸡胚成纤维细胞融合后小鼠1R细胞中次黄嘌呤磷酸核糖基转移酶活性的恢复。

Restoration of hypoxanthine phosphoribosyl transferase activity in mouse 1R cells after fusion with chick-embryo fibroblasts.

作者信息

Bakay B, Croce C M, Koprowski H, Nyhan W L

出版信息

Proc Natl Acad Sci U S A. 1973 Jul;70(7):1998-2002. doi: 10.1073/pnas.70.7.1998.

DOI:10.1073/pnas.70.7.1998
PMID:4516198
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC433651/
Abstract

Fusion of the 1R mouse cell, which lacks activity of hypoxanthine phosphoribosyl transferase (EC 2.4.2.8), with chick-embryo fibroblasts yielded progeny cells that survived in hypoxanthine-aminopterin-thymidine selective medium. This property and the failure of the progeny to survive in 8-azaguanine indicated that hypoxanthine phosphoribosyl transferase activity was present. Electrophoretic analysis revealed that the enzyme was of mouse, not chick, origin. These observations are consistent with the operation of a regulator gene responsible for the absence of hypoxanthine phosphoribosyl-transferase activity in the 1R cell and its presence in the progeny.

摘要

缺乏次黄嘌呤磷酸核糖基转移酶（EC 2.4.2.8）活性的1R小鼠细胞与鸡胚成纤维细胞融合，产生了能在次黄嘌呤 - 氨基蝶呤 - 胸腺嘧啶核苷选择培养基中存活的子代细胞。这一特性以及子代细胞在8 - 氮杂鸟嘌呤中不能存活表明存在次黄嘌呤磷酸核糖基转移酶活性。电泳分析表明该酶起源于小鼠而非鸡。这些观察结果与一个调节基因的作用一致，该调节基因导致1R细胞中缺乏次黄嘌呤磷酸核糖基转移酶活性，而在子代细胞中存在该活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee98/433651/aa4a8c8c3fc7/pnas00070-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee98/433651/ec75332bea8c/pnas00070-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee98/433651/32b73c17e7af/pnas00070-0098-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee98/433651/6f0e418a03b1/pnas00070-0098-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee98/433651/aa4a8c8c3fc7/pnas00070-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee98/433651/ec75332bea8c/pnas00070-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee98/433651/32b73c17e7af/pnas00070-0098-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee98/433651/6f0e418a03b1/pnas00070-0098-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee98/433651/aa4a8c8c3fc7/pnas00070-0099-a.jpg

相似文献

1
Restoration of hypoxanthine phosphoribosyl transferase activity in mouse 1R cells after fusion with chick-embryo fibroblasts.与鸡胚成纤维细胞融合后小鼠1R细胞中次黄嘌呤磷酸核糖基转移酶活性的恢复。
Proc Natl Acad Sci U S A. 1973 Jul;70(7):1998-2002. doi: 10.1073/pnas.70.7.1998.
2
Modulation of the activity of an avian gene transferred into a mammalian cell by cell fusion.通过细胞融合对转入哺乳动物细胞的禽类基因的活性进行调控。
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1398-402. doi: 10.1073/pnas.71.4.1398.
3
Reexpression of the rat hypoxanthine phosphoribosyltransferase gene in rat-human hybrids.大鼠次黄嘌呤磷酸核糖基转移酶基因在大鼠-人杂种细胞中的重新表达。
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2590-4. doi: 10.1073/pnas.70.9.2590.
4
Reappearance of murine hypoxanthine guanine phosphoribosyl transferase activity in mouse A9 cells after attempted hybridisation with human cell lines.在尝试与人类细胞系杂交后，小鼠A9细胞中鼠次黄嘌呤鸟嘌呤磷酸核糖转移酶活性的重现。
Exp Cell Res. 1972 Dec;75(2):401-9. doi: 10.1016/0014-4827(72)90446-6.
5
Reversion in expression of hypoxanthine-guanine phosphoribosyl transferase following cell hybridization.细胞杂交后次黄嘌呤 - 鸟嘌呤磷酸核糖转移酶表达的逆转
J Cell Sci. 1975 May;17(3):567-78. doi: 10.1242/jcs.17.3.567.
6
Evidence for linkage of 3-phosphoglycerate kinase, hypoxanthine-guanine-phosphoribosyl transferase, and glucose 6-phosphate dehydrogenase loci in Chinese hamster cells studied by using a relationship between gene multiplicity and enzyme activity.利用基因多态性与酶活性之间的关系研究中国仓鼠细胞中3-磷酸甘油酸激酶、次黄嘌呤-鸟嘌呤磷酸核糖转移酶和葡萄糖6-磷酸脱氢酶基因座的连锁证据。
Biochem Genet. 1972 Aug;7(1):33-40. doi: 10.1007/BF00487007.
7
Interallelic complementation in hybrid cells derived from Chinese hamster diploid clones deficient in hypoxanthine-guanine phosphoribosyl-transferase activity.源自缺乏次黄嘌呤-鸟嘌呤磷酸核糖转移酶活性的中国仓鼠二倍体克隆的杂交细胞中的等位基因间互补。
Exp Cell Res. 1973 Mar 15;77(1):391-403. doi: 10.1016/0014-4827(73)90593-4.
8
Cytological mapping of human X-linked genes by use of somatic cell hybrids involving an X-autosome translocation (mouse-hamster-human X-linked markers).利用涉及X-常染色体易位的体细胞杂种（小鼠-仓鼠-人类X连锁标记）对人类X连锁基因进行细胞遗传学定位。
Proc Natl Acad Sci U S A. 1972 Jan;69(1):69-73. doi: 10.1073/pnas.69.1.69.
9
Complementation in hybrid cells derived from mutagen-induced mouse clones deficient in hypoxanthine-guanine phosphoribosyl-transferase activity.源自诱变诱导的次黄嘌呤-鸟嘌呤磷酸核糖基转移酶活性缺陷小鼠克隆的杂交细胞中的互补作用。
Exp Cell Res. 1974 Oct;88(2):410-4. doi: 10.1016/0014-4827(74)90261-4.
10
Transfer of genetic information by purified metaphase chromosomes.通过纯化的中期染色体进行遗传信息的转移。
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1258-62. doi: 10.1073/pnas.70.4.1258.

引用本文的文献

1
Monochromosomal Hybrids and Chromosome Transfer: A Functional Approach for Gene Identification.单染色体杂种与染色体转移：一种用于基因鉴定的功能方法。
Cancer Genomics Proteomics. 2017 Mar-Apr;14(2):93-101. doi: 10.21873/cgp.20022.
2
Observations indicating the nature of the mutation in phenylketonuria.表明苯丙酮尿症突变性质的观察结果。
J Inherit Metab Dis. 1980;2(4):79-84. doi: 10.1007/BF01805662.
3
Modulation of the activity of an avian gene transferred into a mammalian cell by cell fusion.通过细胞融合对转入哺乳动物细胞的禽类基因的活性进行调控。

本文引用的文献

1
The separation of adenine and hypoxanthine-guanine phosphoribosyl transferases isoenzymes by disc gel electrophoresis.通过圆盘凝胶电泳分离腺嘌呤和次黄嘌呤 - 鸟嘌呤磷酸核糖基转移酶同工酶。
Biochem Genet. 1971 Feb;5(1):81-90. doi: 10.1007/BF00485733.
2
An improved technique for the separation of glucose 6-phosphate dehydrogenase isoenzymes by disc electrophoresis on polyacrylamide gel.一种通过聚丙烯酰胺凝胶圆盘电泳分离6-磷酸葡萄糖脱氢酶同工酶的改良技术。
Biochem Genet. 1969 Dec;3(6):571-82. doi: 10.1007/BF00485478.
3
Synthesis of an enzyme determined by an erythrocyte nucleus in a hybrid cell.
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1398-402. doi: 10.1073/pnas.71.4.1398.
4
Acquisition of chick cytosol thymidine kinase activity by thymidine kinase-deficient mouse fibroblast cells after fusion with chick erythrocytes.胸苷激酶缺陷型小鼠成纤维细胞与鸡红细胞融合后获得鸡细胞质胸苷激酶活性。
J Cell Biol. 1974 Nov;63(2 Pt 1):505-14. doi: 10.1083/jcb.63.2.505.
5
Discontinuous variability, in the form of a geometric progression, of albumin production in hepatoma and hybrid cells.肝癌细胞和杂交细胞中白蛋白产生呈几何级数形式的间断性变异。
Proc Natl Acad Sci U S A. 1974 May;71(5):2062-6. doi: 10.1073/pnas.71.5.2062.
6
Reexpression of the rat hypoxanthine phosphoribosyltransferase gene in rat-human hybrids.大鼠次黄嘌呤磷酸核糖基转移酶基因在大鼠-人杂种细胞中的重新表达。
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2590-4. doi: 10.1073/pnas.70.9.2590.
7
Adenine phosphoribosyltransferase and hypoxanthine-guanine phosphoribosyltransferase immunoprecipitation reactions in human-mouse and human-hamster cell hybrids.人-鼠和人-仓鼠细胞杂交体中的腺嘌呤磷酸核糖转移酶及次黄嘌呤-鸟嘌呤磷酸核糖转移酶免疫沉淀反应
Humangenetik. 1975 Oct 20;30(1):23-34. doi: 10.1007/BF00273628.
8
Hypoxanthine phosphoribosyltransferase deficiency: association of reduced catalytic activity with reduced levels of immunologically detectable enzyme protein.次黄嘌呤磷酸核糖基转移酶缺乏症：催化活性降低与免疫可检测酶蛋白水平降低的关联。
Proc Natl Acad Sci U S A. 1975 Oct;72(10):4142-6. doi: 10.1073/pnas.72.10.4142.
9
Hypoxanthine-guanine phosphoribosyl transferase deficiency.次黄嘌呤 - 鸟嘌呤磷酸核糖转移酶缺乏症
Hum Genet. 1976 Feb 29;31(2):127-50. doi: 10.1007/BF00296142.
10
Reexpression of HPRT activity following cell fusion with polyethylene glycol.与聚乙二醇细胞融合后次黄嘌呤磷酸核糖基转移酶活性的重新表达。
Biochem Genet. 1978 Apr;16(3-4):227-37. doi: 10.1007/BF00484080.
杂交细胞中由红细胞核决定的一种酶的合成。
J Cell Sci. 1969 Jul;5(1):121-33. doi: 10.1242/jcs.5.1.121.
4
Mitotic separation of two human X-linked genes in man--mouse somatic cell hybrids.人-小鼠体细胞杂种中两个人类X连锁基因的有丝分裂分离
Proc Natl Acad Sci U S A. 1971 Jan;68(1):116-20. doi: 10.1073/pnas.68.1.116.
5
The use of drug-resistant markers to study the hybridization of mouse fibroblasts.使用耐药标记物研究小鼠成纤维细胞的杂交。
Exp Cell Res. 1966 Jan;41(1):190-6. doi: 10.1016/0014-4827(66)90558-1.
6
Detection of radioactive components in polyacrylamide gel disc electropherograms by automated mechanical fractionation.通过自动机械分级分离法检测聚丙烯酰胺凝胶圆盘电泳图谱中的放射性成分。
Anal Biochem. 1971 Apr;40(2):429-39. doi: 10.1016/0003-2697(71)90403-9.
7
Mapping of a human genetic regulator element by somatic cell genetic analysis.通过体细胞遗传学分析对人类遗传调控元件进行定位。
Proc Natl Acad Sci U S A. 1970 Aug;66(4):1220-7. doi: 10.1073/pnas.66.4.1220.
8
Stability of X chromosomal inactivation in human somatic cells.人类体细胞中X染色体失活的稳定性。
Nature. 1972 Sep 8;239(5367):87-9. doi: 10.1038/239087a0.
9
Cytological mapping of human X-linked genes by use of somatic cell hybrids involving an X-autosome translocation (mouse-hamster-human X-linked markers).利用涉及X-常染色体易位的体细胞杂种（小鼠-仓鼠-人类X连锁标记）对人类X连锁基因进行细胞遗传学定位。
Proc Natl Acad Sci U S A. 1972 Jan;69(1):69-73. doi: 10.1073/pnas.69.1.69.
10
Activation of variants of hypoxanthine-guanine phosphoribosyl transferase by the normal enzyme.正常酶对次黄嘌呤 - 鸟嘌呤磷酸核糖基转移酶变体的激活作用。
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2523-6. doi: 10.1073/pnas.69.9.2523.