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鲨鱼产生具有有限异质性的19S IgM抗体。

Production of 19S IgM antibodies with restricted heterogeneity from sharks.

作者信息

Clem L W, Leslie G A

出版信息

Proc Natl Acad Sci U S A. 1971 Jan;68(1):139-41. doi: 10.1073/pnas.68.1.139.

DOI:10.1073/pnas.68.1.139
PMID:5276285
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC391180/
Abstract

Two of three nurse sharks, Ginglymostoma cirratum, immunized with streptococcal A-variant vaccine, produced over 7 mg/ml serum of 19S IgM antibody to the group-specific carbohydrate. The light chains of this specific antibody from one shark were separated into multiple bands by disc electrophoresis. The light chains from the other antibody-producing animal were much less heterogeneous and gave only one predominant band.

摘要

三只护士鲨(长须鲨)中的两只,用A群链球菌变异株疫苗免疫后,产生了超过7毫克/毫升血清的针对群特异性碳水化合物的19S IgM抗体。通过圆盘电泳,来自一条鲨鱼的这种特异性抗体的轻链被分离成多条带。来自另一只产生抗体动物的轻链异质性要小得多,只出现一条主要条带。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e92/391180/e390bed208cc/pnas00076-0147-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e92/391180/da9892ac3266/pnas00076-0147-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e92/391180/22ad69979145/pnas00076-0147-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e92/391180/965cf8b7fb19/pnas00076-0147-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e92/391180/e390bed208cc/pnas00076-0147-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e92/391180/da9892ac3266/pnas00076-0147-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e92/391180/22ad69979145/pnas00076-0147-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e92/391180/965cf8b7fb19/pnas00076-0147-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e92/391180/e390bed208cc/pnas00076-0147-d.jpg

相似文献

1
Production of 19S IgM antibodies with restricted heterogeneity from sharks.鲨鱼产生具有有限异质性的19S IgM抗体。
Proc Natl Acad Sci U S A. 1971 Jan;68(1):139-41. doi: 10.1073/pnas.68.1.139.
2
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Immunoglobulins of the leopard shark. I. Isolation and characterization of 17 S and 7 S immunoglobulins with precipitating activity.豹鲨的免疫球蛋白。I. 具有沉淀活性的17S和7S免疫球蛋白的分离与特性分析。
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引用本文的文献

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Antibody Affinity Maturation in Fishes-Our Current Understanding.鱼类抗体亲和力成熟——我们目前的理解
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3
Evolution and the molecular basis of somatic hypermutation of antigen receptor genes.抗原受体基因的体细胞高频突变的进化与分子基础。

本文引用的文献

1
Studies on the chemical structure of the streptococcal cell wall. I. The identification of a mucopeptide in the cell walls of groups A and A-variant streptococci.链球菌细胞壁化学结构的研究。I. A组和A变异组链球菌细胞壁中一种粘肽的鉴定。
J Exp Med. 1961 Jul 1;114(1):127-40. doi: 10.1084/jem.114.1.127.
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Precipitin activity of rabbit macroglobulin antibody.兔巨球蛋白抗体的沉淀素活性
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Experimental approaches to homogenous antibody populations. Factors controlling the occurrence of antibodies with uniform properties.
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Somatic hypermutation of the new antigen receptor gene (NAR) in the nurse shark does not generate the repertoire: possible role in antigen-driven reactions in the absence of germinal centers.护士鲨新抗原受体基因(NAR)的体细胞超突变不会产生受体库:在没有生发中心的情况下,其在抗原驱动反应中的可能作用。
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Immune response of rats to group A streptococcal vaccine.大鼠对A组链球菌疫苗的免疫反应。
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Phylogenetic conservation of immunoglobulin heavy chains: direct comparison of hamster and mouse Cmu genes.免疫球蛋白重链的系统发育保守性:仓鼠和小鼠Cμ基因的直接比较。
Nucleic Acids Res. 1985 Aug 12;13(15):5611-28. doi: 10.1093/nar/13.15.5611.
获得均质抗体群体的实验方法。控制具有均匀特性抗体产生的因素。
Fed Proc. 1970 Jan-Feb;29(1):59-65.
4
Antibodies of restricted heterogeneity for structural study.用于结构研究的具有受限异质性的抗体。
Fed Proc. 1970 Jan-Feb;29(1):66-71.
5
Phylogeny of immunoglobulin structure and function. II. Immunoglobulins of the nurse shark.
J Immunol. 1967 Dec;99(6):1226-35.
6
Phylogeny of immunoglobulin structure and function. I. Immunoglobulins of the lemon shark.免疫球蛋白结构与功能的系统发育。I. 柠檬鲨的免疫球蛋白
J Exp Med. 1967 May 1;125(5):893-920. doi: 10.1084/jem.125.5.893.
7
Characteristics of streptococcal group-specific antibody isolated from hyperimmune rabbits.从超免疫兔中分离出的链球菌群特异性抗体的特性。
J Exp Med. 1966 Apr 1;123(4):599-614. doi: 10.1084/jem.123.4.599.
8
Horse anti-SI immunoglobulins. I. Properties of gamma-M-antibody.马抗SI免疫球蛋白。I. γ-M抗体的特性。
Biochemistry. 1965 Dec;4(12):2575-84. doi: 10.1021/bi00888a005.
9
Phylogeny of immunoglobulin structure and function. V. Valences and association constants of teleost antibodies to a haptenic determinant.免疫球蛋白结构与功能的系统发育。V. 硬骨鱼抗体与半抗原决定簇的价数和结合常数。
J Exp Med. 1970 Sep 1;132(3):385-400. doi: 10.1084/jem.132.3.385.