Maslow D E, Weiss L
Int J Cancer. 1979 Oct 15;24(4):450-4. doi: 10.1002/ijc.2910240412.
The modification of the tissues surrounding solid tumors is usually attributed exclusively to interactions between the normal tissues and the cancer cells in the tumor, in spite of the fact that tumors contain many different types of non-cancer cells including macrophages. As an experimental model for some facets of the cellular interactions between tumor and normal tissues, we have assessed the individual contributions of macrophages and cancer cells to the differential inhibition of neural retina (NR) cell aggregation by co-culturing NR cells with small numbers of macrophages or cancer cells alone, as well as with both natural (ascites tumors) and artificial mixtures. Cells from human colonic adenocarcinoma inhibited NR aggregation to a greater extent than normal colon mucosa. Aggregation of NR cells was inhibited by macrophages from mice and rats, and to a greater extent by cancer cell suspensions of mouse Ehrlich and rat Walker 256 lines from spinner culture or in the ascites form. Combinations of macrophages and cancer cells indicated that their inhibitory effects were neither additive nor synergistic. Cell-free media from macrophages and cancer cell cultures were equally effective inhibitors of aggregation. The results suggest that the interaction between a malignant tumor and the surrounding normal tissue can be modified by cancer cells, tumor macrophages and their products.
实体瘤周围组织的改变通常完全归因于正常组织与肿瘤中癌细胞之间的相互作用，尽管肿瘤包含许多不同类型的非癌细胞，包括巨噬细胞。作为肿瘤与正常组织之间细胞相互作用某些方面的实验模型，我们通过将神经视网膜（NR）细胞与少量巨噬细胞或癌细胞单独共培养，以及与天然（腹水肿瘤）和人工混合物共培养，评估了巨噬细胞和癌细胞对NR细胞聚集差异抑制的各自贡献。来自人结肠腺癌的细胞比正常结肠黏膜更能抑制NR聚集。小鼠和大鼠的巨噬细胞抑制NR细胞聚集，来自旋转培养或腹水形式的小鼠艾氏腹水瘤和大鼠沃克256细胞系的癌细胞悬液抑制作用更强。巨噬细胞和癌细胞的组合表明它们的抑制作用既不是相加的也不是协同的。来自巨噬细胞和癌细胞培养物的无细胞培养基同样是有效的聚集抑制剂。结果表明，恶性肿瘤与周围正常组织之间的相互作用可被癌细胞、肿瘤巨噬细胞及其产物改变。
