Maslow D E, Mayhew E, Mittleman A
Oncology. 1981;38(5):292-4. doi: 10.1159/000225573.
A model system for studying some aspects of the interaction of cancer cells in tumors and their surrounding nonmalignant tissue is the co-culture of cancer cells and embryonic chick neural retinal cells in gyratory shakers. Neural retina cell aggregation, under these conditions, has been shown to be differentially inhibited by small numbers of cultured mouse and human cancer cells. We extend here these observations to co-cultures of retinal cells with small numbers (60:1 ratio) of human cells isolated from normal colon mucosa or colonic adenocarcinoma tissue. The cells from the malignant tissue samples inhibited aggregation to a significantly greater extent than the cells from normal mucosa, even when both were from the same individual. Cells derived from nonmalignant tumors were more inhibitory than those from normal individuals, which is consistent with described differences in this 'transitional' region. Thus, the aggregation inhibition assay appears applicable to freshly isolated human tissues.
用于研究肿瘤中癌细胞与其周围非恶性组织相互作用某些方面的一个模型系统，是在旋转振荡器中对癌细胞和胚胎鸡神经视网膜细胞进行共培养。在这些条件下，已表明少量培养的小鼠和人类癌细胞会差异性地抑制神经视网膜细胞聚集。我们在此将这些观察结果扩展到视网膜细胞与从正常结肠黏膜或结肠腺癌组织中分离出的少量（60:1比例）人类细胞的共培养。即使来自同一人的恶性组织样本细胞和正常黏膜细胞，前者对聚集的抑制程度也显著大于后者。源自非恶性肿瘤的细胞比来自正常个体的细胞更具抑制性，这与在这个“过渡”区域所描述的差异一致。因此，聚集抑制试验似乎适用于新鲜分离的人类组织。
