对参与兴奋-收缩偶联的心脏超微结构进行体视学测量。
Sterological measurements of cardiac ultrastructures implicated in excitation-contraction coupling.
作者信息
Page E, McCallister L P, Power B
出版信息
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1465-6. doi: 10.1073/pnas.68.7.1465.
Abstract
Electron micrographs of osmium-fixed left ventricles from 200-g female rats were analyzed by stereological techniques. By the use of equations developed by H. Sitte it was possible to determine volume fractions of organelles and absolute membrane areas per unit cell volume for cellular membrane systems implicated in excitation-contraction coupling. The fractions of cell volume were: mitochondria 0.34, myofibrils 0.481, T-system 0.012, total sarcotubules 0.035, other 0.13. The membrane areas per unit cell volume (mum(2)/mum(3)) were: external sarcolemma 0.27, external sarcolemma + T-system 0.34, total sarcotubules 1.3. Diads made up 0.08 of sarcotubular volume and 0.12 of sarcotubular membrane area. 0.14 of the external sarcolemmal membrane area was involved in diadic complexes with underlying subsarcolemmal cisterns.
摘要
运用体视学技术分析了经锇固定的200克雌性大鼠左心室的电子显微照片。通过使用H. Sitte推导的公式，可以确定参与兴奋-收缩偶联的细胞膜系统中细胞器的体积分数以及每单位细胞体积的绝对膜面积。细胞体积的分数分别为：线粒体0.34，肌原纤维0.481，T系统0.012，总肌小管0.035，其他0.13。每单位细胞体积的膜面积（μm²/μm³）分别为：外部肌膜0.27，外部肌膜+T系统0.34，总肌小管1.3。二联体占肌小管体积的0.08和肌小管膜面积的0.12。外部肌膜面积的0.14参与了与潜在肌膜下池形成的二联体复合体。
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