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黑腹果蝇中核的移植

Transplantation of nucle in Drosophila melanogaster.

作者信息

Zalokar M

出版信息

Proc Natl Acad Sci U S A. 1971 Jul;68(7):1539-41. doi: 10.1073/pnas.68.7.1539.

Abstract

Nuclei surrounded by ooplasm of the syncytial stage of developing eggs of wild-type Drosophila melanogaster were implanted into freshly laid fertilized eggs of females of a y w stock. More than half of the recipient eggs produced larvae, but few of the larvae hatched or developed further. The best sets of experiments gave about twelve percent of imagos, mostly y w in appearance. Several larvae were mosaics with yellow Malpighian tubes, and two flies had part of the abdominal segments of the wild type. Half of the flies were fertile, but they produced only y w offspring, except for two males that had y w appearance, but wild-type gonads. When crossed with y w females, they gave wild-type females and y w males.

摘要

将野生型黑腹果蝇发育中卵的合胞体阶段被卵质包围的细胞核,植入yw品系雌蝇刚产下的受精卵中。超过半数的受体卵发育成了幼虫,但只有少数幼虫孵化或进一步发育。最佳的几组实验产生了约12%的成虫,外观大多为yw型。有几只幼虫是带有黄色马尔皮基氏管的嵌合体,还有两只果蝇具有部分野生型腹部节段。一半的果蝇可育,但它们只产生yw型后代,只有两只外观为yw型但具有野生型性腺的雄蝇除外。当与yw型雌蝇杂交时,它们产生了野生型雌蝇和yw型雄蝇。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5966/389235/e9c77f51ef4c/pnas00082-0161-a.jpg

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