Lawrence P A, Johnston P
EMBO J. 1984 Dec 1;3(12):2839-44. doi: 10.1002/j.1460-2075.1984.tb02217.x.
Our purpose is to assess the effects of lethal alleles of engrailed on cells of the internal organs of Drosophila. Using nuclear transplantation we make mosaic flies that contain regions made by engrailed-lethal cells that are genetically labelled. We find that engrailed-lethal cells cause defects in some parts of the epidermis and central nervous system. Most of the internal organs of the fly are assessed and of those, all organs and tissues derived from the endoderm or the splanchnic and somatic mesoderm are normal; flies carrying engrailed-lethal cells in large areas of these organs are viable. We postulate that segments of the mesoderm are single units of cell lineage and that, unlike the ectoderm, they are not subdivided into anterior and posterior compartments.
我们的目的是评估engrailed致死等位基因对果蝇内脏细胞的影响。利用核移植技术，我们培育出了嵌合体果蝇，其中包含由engrailed致死细胞构成且带有遗传标记的区域。我们发现，engrailed致死细胞会导致表皮和中枢神经系统某些部位出现缺陷。我们对果蝇的大部分内脏进行了评估，结果发现，所有源自内胚层或脏壁和体壁中胚层的器官和组织都是正常的；在这些器官的大片区域携带engrailed致死细胞的果蝇能够存活。我们推测，中胚层的各个节段是细胞谱系的单一单元，并且与外胚层不同，它们不会细分为前部和后部区域。
