Cosgrove R F
J Assoc Off Anal Chem. 1979 Sep;62(5):1188-90.
Liquid nitrogen storage of organisms designated in the U.S. Pharmacopeia (XIX) for the assessment of antimicrobial effectiveness is described. By use of simple apparatus and procedures, 85-95% viabilities of pre-frozen cells are attained immediately upon freezing. Viability levels have remained constant over a 3-year period and the use of such inocula has considerably improved the reliability of the test. The superiority of liquid nitrogen storage for microbial test inocular over other types of storage is discussed.
描述了美国药典（第十九版）中指定用于抗菌效力评估的生物体的液氮保存方法。通过使用简单的仪器和程序，预冻细胞在冷冻后立即能达到85% - 95%的存活率。存活率水平在三年期间保持稳定，并且使用这种接种物大大提高了测试的可靠性。讨论了液氮保存用于微生物测试接种物相对于其他保存类型的优越性。
