真核生物起始甲硫氨酰 - tRNA与细菌延伸因子T的真核生物对应物及三磷酸鸟苷的相互作用。
Interaction of eukaryote initiator methionyl-tRNA with the eukaryote equivalent of bacterial elongation factor T and guanosine triphosphate.
作者信息
Richter D, Lipmann F, Tarragó A, Allende J E
出版信息
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1805-9. doi: 10.1073/pnas.68.8.1805.
Abstract
The initiator tRNA, methionyl-tRNA(i) (Met), of yeast and wheat germ forms relatively unstable ternary complexes with their corresponding elongation factors T and GTP. Such complexes can be demonstrated only with fast separation techniques such as Sephadex G-50 and Millipore filtration, but not with the slow Sephadex G-100 method, although both techniques yield stable ternary complexes with all other aminoacyl-tRNAs, including the internal Met-tRNA(m) (Met). To bind yeast-initiating Met-tRNA(i) (Met) to ribosomes, initiation factors present in a ribosomal wash fraction from yeast are needed.
摘要
酵母和小麦胚芽的起始tRNA，即甲硫氨酰 - tRNA(i)（Met），与其相应的延伸因子T和GTP形成相对不稳定的三元复合物。只有通过诸如葡聚糖G - 50和微孔过滤等快速分离技术才能证明此类复合物的存在，而不能用较慢的葡聚糖G - 100方法，尽管这两种技术都能与包括内部Met - tRNA(m)（Met）在内的所有其他氨酰 - tRNA形成稳定的三元复合物。为了使酵母起始Met - tRNA(i)（Met）与核糖体结合，需要酵母核糖体洗涤组分中存在的起始因子。
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2
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3
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