真核生物延伸因子EF 1与鸟苷核苷酸及氨酰-tRNA的相互作用。
Interaction of eukaryote elongation factor EF 1 with guanosine nucleotides and aminoacyl-tRNA.
作者信息
Moon H M, Redfield B, Weissbach H
出版信息
Proc Natl Acad Sci U S A. 1972 May;69(5):1249-52. doi: 10.1073/pnas.69.5.1249.
Abstract
Evidence for two species of elongation factor 1 (EF 1(A) and EF 1(B)) from calf brain has been obtained by molecular sieve chromatography on Sephadex G-150. A high molecular weight form, EF 1(A), interacts with GTP to form an EF 1(A)-GTP complex. GDP also reacts with EF 1, but unlike the reaction with GTP, an EF 1(B)-GDP complex is formed that contains a lower molecular weight and labile species of EF 1. The results also indicate that EF 1(A)-GTP reacts with aminoacyl-tRNA to form an aminoacyl-tRNA-EF 1(B)-GTP complex. These results are discussed with regard to the role of EF 1 in aminoacyl-tRNA binding to ribosomes.
摘要
通过在葡聚糖凝胶G - 150上进行分子筛层析，已获得来自小牛脑的两种延伸因子1（EF 1(A)和EF 1(B)）的证据。一种高分子量形式的EF 1(A)与GTP相互作用形成EF 1(A)-GTP复合物。GDP也与EF 1反应，但与和GTP的反应不同，会形成一种EF 1(B)-GDP复合物，其包含分子量较低且不稳定的EF 1种类。结果还表明，EF 1(A)-GTP与氨酰 - tRNA反应形成氨酰 - tRNA - EF 1(B)-GTP复合物。针对EF 1在氨酰 - tRNA与核糖体结合中的作用对这些结果进行了讨论。
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