Akizuki M, Powers R, Holman H R
J Clin Invest. 1977 Feb;59(2):264-72. doi: 10.1172/JCI108637.
A soluble nuclear antigen that reacts with sera obtained from patients with systemic lupus erythematosus and Sjögren's syndrome has been described. The antigen, tentatively named the Ha antigen after the prototype serum, was shown to react with specific antibodies by precipitin, complement fixation, and immunofluorescence techniques. The Ha antigen prepared from isolated nuclei of calf thymus glands, calf liver, and rat liver showed identical immunological reactivities; a wide distribution among different species and tissues is presumed. The Ha antigen was destroyed by trypsin and relatively mild heat or pH variation from neutrality, but was resistant to DNase or RNase. Many of these characteristics are similar to those of the "B" antigen to which antibodies have recently been described in Sjögren's syndrome. The nuclear origin of the Ha antigen was confirmed by the speckled nuclear immunofluorescence staining pattern given by purified antibody to Ha obtained from a specific immune precipitate. Preliminary results showed approximately 13% of patients with systemic lupus erythematosus and 30% of patients with Sjögren's syndrome had precipitating antibodies to the Ha antigen.
已描述了一种可溶核抗原，它能与系统性红斑狼疮和干燥综合征患者的血清发生反应。该抗原根据原型血清暂命名为Ha抗原，通过沉淀素、补体结合和免疫荧光技术显示其能与特异性抗体发生反应。从小牛胸腺、小牛肝脏和大鼠肝脏的分离核中制备的Ha抗原显示出相同的免疫反应性；推测其在不同物种和组织中广泛分布。Ha抗原可被胰蛋白酶以及相对温和的加热或偏离中性的pH变化破坏，但对DNA酶或RNA酶有抗性。这些特征中有许多与干燥综合征中最近描述的有抗体的“B”抗原相似。通过从特异性免疫沉淀物中获得的纯化抗Ha抗体产生的斑点状核免疫荧光染色模式，证实了Ha抗原的核起源。初步结果显示，约13%的系统性红斑狼疮患者和30%的干燥综合征患者有针对Ha抗原的沉淀抗体。
