真核生物核糖体的大小变化。
Size changes in eukaryotic ribosomes.
作者信息
Vournakis J, Rich A
出版信息
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3021-5. doi: 10.1073/pnas.68.12.3021.
Abstract
Evidence is presented that ribosomes active in protein synthesis and attached to messenger RNA on polysomes have a smaller diameter than free cytoplasmic single ribosomes. Measurements have been made on these two types of ribosomes of differences in sedimentation velocity and diffusion constant. Differences in these quantities suggest about a 20-A decrease in the diameter of the ribosomes from chick embryo muscles when they are attached to messenger RNA. Similar differences are also observed in rabbit reticulocytes and mouse ascites tumor cells. These two ribosomal states have different sensitivity to Pronase digestion and dissociate into ribosomal subunits at different KCl concentrations. This size difference is not associated with a significant difference in overall ribosomal mass and appears not to be dependent upon the presence of nascent polypeptide chains.
摘要
有证据表明，在蛋白质合成中活跃并附着于多聚核糖体上信使核糖核酸的核糖体，其直径比游离于细胞质中的单个核糖体小。已对这两种核糖体的沉降速度和扩散常数差异进行了测量。这些量的差异表明，鸡胚肌肉中的核糖体在附着于信使核糖核酸时，其直径大约减小了20埃。在兔网织红细胞和小鼠腹水肿瘤细胞中也观察到了类似差异。这两种核糖体状态对链霉蛋白酶消化的敏感性不同，并且在不同的氯化钾浓度下解离为核糖体亚基。这种大小差异与核糖体整体质量的显著差异无关，而且似乎不依赖于新生多肽链的存在。
相似文献
1
Size changes in eukaryotic ribosomes.真核生物核糖体的大小变化。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3021-5. doi: 10.1073/pnas.68.12.3021.
2
Ribosome crystallization in chicken embryos. I. Isolation, characterization, and in vitro activity of ribosome tetramers.鸡胚中的核糖体结晶。I. 核糖体四聚体的分离、表征及体外活性
J Cell Biol. 1972 Feb;52(2):338-54. doi: 10.1083/jcb.52.2.338.
3
Globin mRNA from rabbit reticulocyte membrane-bound ribosomes.来自兔网织红细胞膜结合核糖体的珠蛋白信使核糖核酸
Biochim Biophys Acta. 1975 Jan 6;378(1):73-9. doi: 10.1016/0005-2787(75)90138-0.
4
Resistance of messenger RNA-bound ribosomes to proteolytic dissociation.
Exp Cell Res. 1972 Jun;72(2):431-5. doi: 10.1016/0014-4827(72)90011-0.
5
Separation and translation of the mRNAs coding for and chains of rabbit globin.编码兔珠蛋白α链和β链的信使核糖核酸（mRNA）的分离与转译
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1574-7. doi: 10.1073/pnas.69.6.1574.
6
Studies on a nucleoprotein prepared from rat liver polysomes by digestion with T1 ribonuclease.关于用T1核糖核酸酶消化大鼠肝脏多核糖体所制备的一种核蛋白的研究。
Biochem J. 1969 Jul;113(4):643-50. doi: 10.1042/bj1130643.
7
Myosin heavy chain messenger RNA from myogenic cell cultures.来自成肌细胞培养物的肌球蛋白重链信使核糖核酸
Proc Natl Acad Sci U S A. 1974 Mar;71(3):662-6. doi: 10.1073/pnas.71.3.662.
8
Messenger RNA associated with polysomes, ribosomes and subribosomal particles in KB cells.与KB细胞中的多核糖体、核糖体及核糖体亚颗粒相关的信使核糖核酸。
Biochim Biophys Acta. 1967 Jun 20;142(1):65-74. doi: 10.1016/0005-2787(67)90515-1.
9
Preferential degradation of "messenger RNA" in reticulocytes by ribonuclease treatment and sonication of polysomes.
Nature. 1969 Aug 9;223(5206):628-30. doi: 10.1038/223628a0.
10
Selective proteolytic dissociation of rabbit reticulocyte single ribosomes not attached to messenger RNA.兔网织红细胞中未附着信使核糖核酸的单个核糖体的选择性蛋白水解解离
Biochim Biophys Acta. 1971 Feb 11;228(3):719-27. doi: 10.1016/0005-2787(71)90736-2.
引用本文的文献
1
Heuristic Modeling and 3D Stereoscopic Visualization of a Chlamydomonas reinhardtii Cell.莱茵衣藻细胞的启发式建模与3D立体可视化
J Integr Bioinform. 2018 Jul 11;15(2):/j/jib.2018.15.issue-2/jib-2018-0003/jib-2018-0003.xml. doi: 10.1515/jib-2018-0003.
2
The helicase protein DHX29 promotes translation initiation, cell proliferation, and tumorigenesis.解旋酶蛋白 DHX29 可促进翻译起始、细胞增殖和肿瘤发生。
Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22217-22. doi: 10.1073/pnas.0909773106. Epub 2009 Dec 11.
3
Effect of light on ribonucleic Acid metabolism in greening maize leaves.光照对绿玉米叶片中核糖核酸代谢的影响。
Plant Physiol. 1973 Jan;51(1):10-6. doi: 10.1104/pp.51.1.10.
4
Studies of ribosomal diffusion coefficients using laser light-scattering spectroscopy.利用激光散射光谱法对核糖体扩散系数的研究。
Biophys J. 1974 Jul;14(7):528-45. doi: 10.1016/S0006-3495(74)85933-3.
本文引用的文献
1
On the equilibrium of the association-dissociation reaction of ribosomal subparticles and on the existance of the so-called '60 S intermediate' ('swollen 70 S') during centrifugation of the equilibrium mixture.关于核糖体亚颗粒缔合-解离反应的平衡以及平衡混合物离心过程中所谓“60S中间体”（“肿胀的70S”）的存在。
FEBS Lett. 1971 May 20;14(5):349-353. doi: 10.1016/0014-5793(71)80298-3.
2
Buoyant densities of cytoplasmic ribonucleoprotein particles of mammalian cells: distinctive character of ribosome subunits and the rapidly labeled components.哺乳动物细胞胞质核糖核蛋白颗粒的浮力密度：核糖体亚基和快速标记成分的独特特征。
J Mol Biol. 1966 Apr;16(2):255-68. doi: 10.1016/s0022-2836(66)80171-7.
3
The ribosomal subunit--polyribosome cycle in protein synthesis of embryonic skeletal muscle.胚胎骨骼肌蛋白质合成中的核糖体亚基-多核糖体循环
Biochemistry. 1969 Sep;8(9):3742-9. doi: 10.1021/bi00837a038.
4
The role of ribosomal subunits and 80-S monomers in polysome formation in an ascites tumour cell.核糖体亚基和80-S单体在腹水肿瘤细胞多核糖体形成中的作用。
Biochim Biophys Acta. 1968 Nov 20;169(1):139-49. doi: 10.1016/0005-2787(68)90015-4.
5
A study of muscle polyribosomes and the coprecipitation of polyribosomes with myosin.一项关于肌肉多核糖体以及多核糖体与肌球蛋白共沉淀的研究。
Biochemistry. 1968 Sep;7(9):3289-96. doi: 10.1021/bi00849a036.
6
Molecular weights of coliphages and colip- hage DNA. II. Measurement of diffusion coefficients using optical mixing spectroscopy, and measurement of sedimentation coefficients.大肠杆菌噬菌体和大肠杆菌噬菌体DNA的分子量。II. 使用光学混合光谱法测量扩散系数以及测量沉降系数。
J Mol Biol. 1970 Dec 28;54(3):547-56. doi: 10.1016/0022-2836(70)90125-7.
7
Structure and function of mammalian ribosomes. I. Isolation and characterization of active liver ribosomal subunits.哺乳动物核糖体的结构与功能。I. 活性肝脏核糖体亚基的分离与特性分析。
J Mol Biol. 1970 Oct 14;53(1):1-19. doi: 10.1016/0022-2836(70)90042-2.
8
Resistance of active yeast ribosomes to dissociation by KCl.
J Biol Chem. 1970 Mar 25;245(6):1504-6.
9
Nascent subribosomal particles in Tetrahymena pyriformis.梨形四膜虫中的新生亚核糖体颗粒。
Eur J Biochem. 1970 Apr;13(2):238-46. doi: 10.1111/j.1432-1033.1970.tb00923.x.
10
Effect of sodium fluoride on protein synthesis in HeLa cells: inhibition of ribosome dissociation.氟化钠对HeLa细胞蛋白质合成的影响：抑制核糖体解离。
J Mol Biol. 1970 Feb 14;47(3):335-52. doi: 10.1016/0022-2836(70)90306-2.
Zotero 插件