通过剥夺血清或锌来抑制鸡胚培养物中的DNA合成。
Inhibition of DNA synthesis in chick embryo cultures by deprivation of either serum or zinc.
作者信息
Rubin H, Koide T
出版信息
J Cell Biol. 1973 Mar;56(3):777-86. doi: 10.1083/jcb.56.3.777.
The rate of DNA synthesis in cultures of chick embryo cells is proportional to the concentration of serum added. The concentration of serum required to stimulate DNA synthesis increases with cell population density and with the duration of culture after trypsinization. The increase of the serum requirement with population density is not caused by the depletion of serum constituents. The requirement of cells for external zinc in DNA synthesis also increases with population density and duration of culture. The kinetics of inhibition of DNA synthesis by deprivation of serum or zinc are similar. Serum deprivation, however, inhibits 2-deoxyglucose uptake and cell movement, but zinc deprivation does not. The deprivation of either serum or zinc inhibits RNA synthesis about twofold. Very low concentrations of actinomycin D prevent the resumption of RNA and DNA synthesis upon restoration of serum or zinc to deprived cultures.
鸡胚细胞培养物中DNA合成的速率与添加血清的浓度成正比。刺激DNA合成所需的血清浓度随着细胞群体密度以及胰蛋白酶消化后培养时间的延长而增加。血清需求随群体密度增加并非由血清成分的耗尽所致。细胞在DNA合成中对外部锌的需求也随群体密度和培养时间的延长而增加。血清或锌缺乏对DNA合成的抑制动力学相似。然而，血清缺乏会抑制2-脱氧葡萄糖摄取和细胞运动，而锌缺乏则不会。血清或锌缺乏均会使RNA合成受到约两倍的抑制。极低浓度的放线菌素D可阻止在向缺乏血清或锌的培养物中恢复血清或锌后RNA和DNA合成的恢复。
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