用于分枝杆菌噬菌体分型的培养基和方法。
Media and methods for phage-typing mycobacteria.
作者信息
Redmond W B, Ward D M
出版信息
Bull World Health Organ. 1966;35(4):563-8.
Abstract
Media widely used for primary isolation, for drug-sensitivity tests and for type differentiation of mycobacteria have been tested for use in phage studies on the tubercle bacilli. Modifications of each of these media provided some improvement in phage activity on some strains of mycobacteria but not on others. During these studies it was observed that certain substances augmented phage activity, while other substances were found to have the opposite effect. On the basis of these results, a new medium has been formulated. This medium can be used without agar as a liquid medium for growth of the mycobacteria or with agar as a solid medium for isolated plaques and routine test dilution (RTD) spots. An outline of a pilot phage-typing test on three species of Mycobacterium is included.
摘要
已对广泛用于分枝杆菌初次分离、药敏试验及分型的培养基进行了测试，以用于结核杆菌的噬菌体研究。对这些培养基中的每一种进行改良后，在某些分枝杆菌菌株上，噬菌体活性有了一定提高，但在其他菌株上却没有。在这些研究过程中发现，某些物质可增强噬菌体活性，而其他物质则有相反作用。基于这些结果，已配制出一种新培养基。这种培养基可不加琼脂用作分枝杆菌生长的液体培养基，也可加琼脂用作分离噬菌斑和常规试验稀释度（RTD）斑点的固体培养基。文中还包括了对三种分枝杆菌进行噬菌体分型初步试验的概述。
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