Gangadharam P R, Simmons C S, Stager C E
Am Rev Respir Dis. 1978 Jul;118(1):148-50. doi: 10.1164/arrd.1978.118.1.148.
The suitability of phage-impregnated paper discs for the phage typing of mycobacteria was studied. The relevance of the routine test dilution, the volume of the phage used, the mode of incubation, and the effect of prolonged storage of phage-impregnated paper discs were considered. By using paper discs, each impregnated with one of 5 different mycobacteriophages (BG1, BK1, G37, CRI-3 and LG) that lyse Mycobacterium smegmatis 607B, it was determined that 100 x the routine test dilution in a volume of at least 20 microliter was required for phage lysis. Soaked and dried paper discs produced larger areas of lysis than those with 20-microliter volumes. Soaked discs were found to be stable even after storage for 8 weeks at 4 degrees C.
研究了噬菌体浸渍纸片用于分枝杆菌噬菌体分型的适用性。考虑了常规试验稀释度、所用噬菌体的体积、孵育方式以及噬菌体浸渍纸片长期储存的影响。通过使用分别浸渍有5种不同分枝杆菌噬菌体（BG1、BK1、G37、CRI - 3和LG）之一的纸片，这些噬菌体可裂解耻垢分枝杆菌607B，结果确定噬菌体裂解需要在至少20微升体积中使用常规试验稀释度的100倍。浸泡并干燥的纸片产生的裂解区域比20微升体积的纸片更大。发现浸泡后的纸片即使在4℃储存8周后仍保持稳定。
