Zoon K C, Habersat M, Scocca J J
J Bacteriol. 1976 Jul;127(1):545-54. doi: 10.1128/jb.127.1.545-554.1976.
Six polypeptides with apparent molecular weights of 95,000, 90,000, 80,000, 67,000, 64,000, and 43,000 were found to be characteristic of the cell envelopes of competent Haemophilus influenzae, and were synthesized entirely during the period of competence development. Two polypeptides with apparent molecular weights of 58,500 and 40,500 were synthesized during growth as well as during competence development, but were only associated with the envelope fraction of cells that had developed competence. The kinetics of synthesis of the competence-related envelope polypeptides showed a lag period of approximately 20 min. The observation of this lag period raises the question as to whether some of these competence-related polypeptides might be involved in the process of deoxyribonucleic acid uptake, since the development of this property also exhibits a sigmoid time course during competence development.
发现六种表观分子量分别为95,000、90,000、80,000、67,000、64,000和43,000的多肽是感受态流感嗜血杆菌细胞膜的特征性成分，并且完全在感受态发育期间合成。两种表观分子量分别为58,500和40,500的多肽在生长以及感受态发育期间均有合成，但仅与已发育出感受态的细胞的包膜部分相关。感受态相关包膜多肽的合成动力学显示约20分钟的延迟期。这一延迟期的观察引发了一个问题，即这些感受态相关多肽中的一些是否可能参与脱氧核糖核酸摄取过程，因为这种特性的发展在感受态发育期间也呈现出S形时间进程。
