小鼠L细胞中的红霉素抗性

Erythromycin resistance in mouse L cells.

作者信息

Molloy P L, Eisenstadt J M

出版信息

Somatic Cell Genet. 1979 Sep;5(5):585-95. doi: 10.1007/BF01542696.

Abstract

The sensitivity of mouse cell lines in culture to the macrolide antibiotic, erythromycin stearate, was investigated. Both resistant and sensitive lines were found. Experiments indicated that in sensitive cells erythromycin stearate inhibits mitochondrial protein synthesis. Mutants resistant to erythromycin stearate were selected from the line LM(TK-), and these are also less sensitive to other macrolide antibiotics such as carbomycin and spiramycin. Attempts to transfer the erythromycin resistance of either the mutants or naturally resistant lines by fusion of cytoplasts with sensitive cells were unsuccessful, and it is concluded that resistance to erythromycin stearate is controlled by nuclear genetic factors.

摘要

研究了培养的小鼠细胞系对大环内酯类抗生素硬脂酸红霉素的敏感性。发现了抗性和敏感细胞系。实验表明，在敏感细胞中，硬脂酸红霉素抑制线粒体蛋白质合成。从LM(TK-)细胞系中筛选出对硬脂酸红霉素有抗性的突变体，这些突变体对其他大环内酯类抗生素如碳霉素和螺旋霉素也较不敏感。通过将细胞质体与敏感细胞融合来转移突变体或天然抗性细胞系对红霉素的抗性的尝试未成功，得出结论：对硬脂酸红霉素的抗性由核遗传因素控制。