Ambekar G R, Thadani S B, Doctor V M
Appl Microbiol. 1965 Sep;13(5):713-9. doi: 10.1128/am.13.5.713-719.1965.
The waste mycelium of Penicillium chrysogenum HA-10 (obtained at the end of penicillin fermentation), or a 24-hr-old freshly grown vegetative inoculum of this organism, was found to utilize glucose for the production of calcium gluconate by submerged fermentation in shake flasks. After 72 to 96 hr of fermentation at 24 C, 90 to 95% of the reducing sugar from the 15% glucose medium was converted to calcium gluconate. Reuse of the mycelium for successive experiments reduced the fermentation period to 72 hr or less because of an enhancement of glucose utilization. Ten successive batches of 15% glucose medium were fermented by the reuse method. Fermentation media containing up to 30% glucose could be used, provided boric acid was added to prevent the precipitation of calcium gluconate formed. We found that 30% hydrol (a by-product of glucose manufacture containing 50 to 55% reducing sugar), when used in place of glucose in the fermentation medium, inhibited the rate of glucose utilization. However, this effect was partially reversed by pretreatment of hydrol with 2 to 4% activated charcoal before addition to the fermentation medium.
产黄青霉HA-10的废弃菌丝体（在青霉素发酵结束时获得），或该生物体24小时龄的新鲜生长的营养接种物，被发现可通过摇瓶中的深层发酵利用葡萄糖来生产葡萄糖酸钙。在24℃下发酵72至96小时后，15%葡萄糖培养基中90%至95%的还原糖转化为葡萄糖酸钙。由于葡萄糖利用率提高，将菌丝体重复用于后续实验可将发酵时间缩短至72小时或更短。采用重复利用方法对15%葡萄糖培养基进行了十批连续发酵。只要添加硼酸以防止形成的葡萄糖酸钙沉淀，就可以使用含高达30%葡萄糖的发酵培养基。我们发现，当在发酵培养基中使用30%的水解糖（葡萄糖制造的副产品，含有50%至55%的还原糖）代替葡萄糖时，会抑制葡萄糖的利用速率。然而，在将水解糖添加到发酵培养基之前，用2%至4%的活性炭对其进行预处理可部分逆转这种影响。
