Poonawalla F M, Patel K L, Iyengar M R
Appl Microbiol. 1965 Sep;13(5):749-54. doi: 10.1128/am.13.5.749-754.1965.
A study was made of Penicillium chrysogenum and some other fungi to determine the relative distribution of intra- and extracellular invertase produced by them in submerged fermentation. The proportion of each type of enzyme varied with the organism and the period of fermentation. More of the enzyme initially bound to the mycelium was released into the medium with the progress of fermentation. Differences were observed in the effects of cultural conditions on enzyme production in P. chrysogenum and Saccharomyces cerevisiae. Considerably greater quantities of enzyme were produced by P. chrysogenum and the yeast in both laboratory and large-scale fermentors when sucrose was added continuously than when the same quantities of the sugar were added initially.
对产黄青霉和其他一些真菌进行了一项研究，以确定它们在深层发酵中产生的胞内和胞外转化酶的相对分布。每种酶的比例随微生物和发酵时间而变化。随着发酵的进行，最初与菌丝体结合的酶更多地释放到培养基中。观察到培养条件对产黄青霉和酿酒酵母中酶产生的影响存在差异。当连续添加蔗糖时，与最初添加相同量的糖相比，产黄青霉和酵母在实验室和大型发酵罐中产生的酶量要多得多。
