针对相同抗原决定簇的B淋巴细胞和T淋巴细胞上的共享独特型决定簇。III. 使用抗独特型抗体通过亲和层析对特异性免疫活性T淋巴细胞进行物理分离。

Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. III. Physical fractionation of specific immunocompetent T lymphocytes by affinity chromatography using anti-idiotypic antibodies.

作者信息

Binz H, Wigzell H

出版信息

J Exp Med. 1975 Nov 1;142(5):1231-40. doi: 10.1084/jem.142.5.1231.

DOI:10.1084/jem.142.5.1231

PMID:53260

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2189975/

Abstract

Anti-idiotypic antibodies made against antigen-binding receptors on T lymphocytes with specificity for certain Ag-B locus antigens selectively react with T lymphocytes with potential immune reactivity against the very same Ag-B antigens. This was shown by affinity chromatography of normal Lewis T lymphocytes on anti-Ig columns after contact with the relevant anti-idiotypic antiserum. Here, it could be shown that incubation of the cells with an anti-(Lewis-anti-BN) antiserum caused subsequent selective retention of potential graft-vs.-host (GvH)-reactive cells against BN on the anti-Ig column, whereas Lewis T cells with reactivity against DA or August (Au) (carrying distinct Ag-B antigens in comparison to BN) passed through. The retained cells could be eluted and shown to display highly increased reactivity against BN with virtually no reactivity left against DA or Au antigens. Analogous results were obtained using an anti-(Lewis-anti-DA) antiserum. The anti-idiotypic antibodies can be used in fluorescent antibody tests to directly visualize the idiotype-positive cells. Using the separation design described above we analyzed selectively enriched or deleted T lymphocytes for presence of idiotypic cells as well as specific GvH reactivity. A highly significant positive correlation was found between percentage of a given idiotype in a population of T cells and the relevant GvH potential of the same T cells that can be visualized are indeed the very same T cells that express immune reactivity against the expected antigens. The present data would thus directly demonstrate the existence of a largely nonoverlapping population of immunocompetent T cells capable of reacting against the various Ag-B locus antigens in the rat. Highly purified, functionally intact immunocompetent T lymphocytes with restricted immune reactivity can thus be produced from normal lymphocyte populations for further analysis.

摘要

针对T淋巴细胞上抗原结合受体产生的、对某些Ag - B位点抗原具有特异性的抗独特型抗体，能选择性地与对相同Ag - B抗原具有潜在免疫反应性的T淋巴细胞发生反应。这一点通过正常Lewis T淋巴细胞在与相关抗独特型抗血清接触后，在抗Ig柱上进行亲和层析得以证明。在此可以表明，用抗（Lewis - 抗 - BN）抗血清孵育细胞后，随后对BN具有潜在移植物抗宿主（GvH）反应性的细胞会选择性地保留在抗Ig柱上，而对DA或奥古斯特（Au）（与BN相比携带不同的Ag - B抗原）具有反应性的Lewis T细胞则通过柱子流出。保留的细胞可以被洗脱，并显示出对BN的反应性大幅增加，而对DA或Au抗原几乎没有反应性。使用抗（Lewis - 抗 - DA）抗血清也获得了类似的结果。抗独特型抗体可用于荧光抗体检测，以直接可视化独特型阳性细胞。使用上述分离设计，我们分析了选择性富集或缺失的T淋巴细胞中独特型细胞的存在情况以及特异性GvH反应性。在T细胞群体中给定独特型的百分比与同一T细胞的相关GvH潜力之间发现了高度显著的正相关，这些可被可视化的T细胞确实就是对预期抗原表现出免疫反应性的同一批T细胞。因此，目前的数据将直接证明在大鼠中存在大量基本不重叠的、能够对各种Ag - B位点抗原产生反应的免疫活性T细胞群体。因此，可以从正常淋巴细胞群体中产生具有受限免疫反应性的高度纯化、功能完整的免疫活性T淋巴细胞，用于进一步分析。

相似文献

Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. III. Physical fractionation of specific immunocompetent T lymphocytes by affinity chromatography using anti-idiotypic antibodies.针对相同抗原决定簇的B淋巴细胞和T淋巴细胞上的共享独特型决定簇。III. 使用抗独特型抗体通过亲和层析对特异性免疫活性T淋巴细胞进行物理分离。

J Exp Med. 1975 Nov 1;142(5):1231-40. doi: 10.1084/jem.142.5.1231.

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In vitro induction of specific unresponsiveness of immunologically reactive, normal bone marrow cells.体外诱导免疫活性正常骨髓细胞的特异性无反应性。

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On the relationship between cellular and humoral antibodies.关于细胞抗体与体液抗体之间的关系。

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Cell-bound receptors for alloantigens on normal lymphocytes. I. Characterization of receptor-carrying cells by the use of antibodies to alloantibodies.正常淋巴细胞上同种异体抗原的细胞结合受体。I. 利用抗同种抗体的抗体对携带受体的细胞进行特性鉴定。

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Proc Natl Acad Sci U S A. 1972 Jun;69(6):1620-4. doi: 10.1073/pnas.69.6.1620.

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