Lederberg S
J Bacteriol. 1966 Mar;91(3):1029-36. doi: 10.1128/jb.91.3.1029-1036.1966.
Lederberg, Seymour (Brown University, Providence, R.I.). Genetics of host-controlled restriction and modification of deoxyribonucleic acid in Escherichia coli. J. Bacteriol. 91:1029-1036. 1966.-The locus for the host specific restriction and modification of deoxyribonucleic acid in Escherichia coli has been mapped by matings between mutants for these characters in strains K-12, C600, and B. Linkage analysis and kinetics of marker transfer indicate that a single or closely linked multiple chromosomal site located about 4 min counterclockwise to leucine is responsible for these activities. Secondary factors which affect the quantitative level of restriction also were detected. Wild-type recombinants were isolated in crosses between rm(-) (restriction or modification, or both) mutants. The expression in zygotes of the restrictionless character of a rm(-) donor is masked by a separate, physiological impairment of restriction, which results from mating and is independent of the modification state of the donor. The relevance of the restriction character to mating incompatibilities in these and other bacterial strains is considered.
莱德伯格，西摩（布朗大学，罗德岛普罗维登斯）。大肠杆菌中宿主控制的脱氧核糖核酸限制与修饰的遗传学。《细菌学杂志》91:1029 - 1036。1966年。——通过K - 12、C600和B菌株中这些性状的突变体之间的交配，对大肠杆菌中宿主特异性脱氧核糖核酸限制与修饰的基因座进行了定位。连锁分析和标记转移动力学表明，位于亮氨酸逆时针方向约4分钟处的一个单一或紧密连锁的多个染色体位点负责这些活性。还检测到了影响限制定量水平的次要因素。在rm（ - ）（限制或修饰，或两者皆无）突变体之间的杂交中分离出了野生型重组体。rm（ - ）供体的无限制性状在合子中的表达被一种单独的、由交配引起且与供体修饰状态无关的生理性限制损伤所掩盖。讨论了这些及其他细菌菌株中限制性状与交配不相容性的相关性。
