细菌核糖体对核糖体核糖核酸酶的抑制作用。
The inhibition of ribosomal ribonuclease by bacterial ribosomes.
作者信息
Wade H E, Robinson H K
出版信息
Biochem J. 1965 Dec;97(3):747-53. doi: 10.1042/bj0970747.
Abstract
- A comparison has been made between the ribonuclease activities of untreated ribosomes from Escherichia coli B and Pseudomonas fluorescens and the activities of ribosomes on to which ribosomal ribonuclease from E. coli B has been adsorbed. 2. The normal ribosomes from both species were stable in 5-10mm-Mg(2+) (I0.16) at pH6. The RNA in ribosomes from Ps. fluorescens was attacked by the adsorbed ribonuclease under these conditions, whereas the ribosomes from E. coli B were able to adsorb and inhibit this enzyme. 3. Inhibition was also observed with ribosomes from Aerobacter aerogenes, Proteus vulgaris and two other strains of E. coli. It was not observed in ribosomes from three species of Pseudomonas. 4. The inhibition depended on the integrity of the ribosomes and was not observed under conditions of low Mg(2+) concentration that cause irreversible degradation into more slowly sedimenting particles.
摘要
- 对来自大肠杆菌B和荧光假单胞菌的未处理核糖体的核糖核酸酶活性，以及吸附了来自大肠杆菌B的核糖体核糖核酸酶的核糖体活性进行了比较。2. 两种菌的正常核糖体在pH6的5 - 10mM - Mg(2+)（离子强度0.16）中是稳定的。在这些条件下，荧光假单胞菌核糖体中的RNA受到吸附的核糖核酸酶攻击，而大肠杆菌B的核糖体能够吸附并抑制这种酶。3. 在产气气杆菌、普通变形杆菌和另外两株大肠杆菌的核糖体中也观察到了抑制作用。在三种假单胞菌的核糖体中未观察到这种抑制作用。4. 这种抑制作用取决于核糖体的完整性，在低Mg(2+)浓度导致不可逆降解为沉降较慢的颗粒的条件下未观察到这种抑制作用。
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