Ganesan A K, Rotman B
J Bacteriol. 1966 Nov;92(5):1378-82. doi: 10.1128/jb.92.5.1378-1382.1966.
Ganesan, Ann K. (Syntex Institute of Molecular Biology, Palo Alto, Calif.), and Boris Rotman. Transfer and incorporation of genes controlling beta-d-galactosidase synthesis from Hfr and F' donors of Escherichia coli. J. Bacteriol. 92:1378-1382. 1966.-Comparisons were made between Hfr(1) and F(13) donors with respect to the frequency of transfer and incorporation of genes controlling beta-d-galactosidase synthesis. The Hfr(1) donor transfers these genes as part of the chromosome, and the F(13) donor transfers them by F-duction. The criterion used for gene transfer was the acquisition by recipient cells of the ability to synthesize the enzyme, beta-d-galactosidase, measured by fluorogenic assays at the single-cell level. The criterion for incorporation was the formation of lac(+) recombinant colonies. It was found that the two types of donor showed the same frequency of gene transfer, but the probability of incorporation was 10-fold higher in F(13) matings than in Hfr(1) matings. In the former, between 46 and 97% of the merozygotes produced recombinant colonies; in the latter, 2 to 6% did so.
甘内桑，安·K.（加利福尼亚州帕洛阿尔托市辛泰克斯分子生物学研究所），以及鲍里斯·罗特曼。来自大肠杆菌Hfr和F'供体的控制β - d - 半乳糖苷酶合成基因的转移与整合。《细菌学杂志》92:1378 - 1382。1966年。——就控制β - d - 半乳糖苷酶合成基因的转移频率和整合频率，对Hfr(1)和F(13)供体进行了比较。Hfr(1)供体将这些基因作为染色体的一部分进行转移，而F(13)供体通过F因子转导进行转移。用于基因转移的标准是受体细胞获得合成β - d - 半乳糖苷酶这种酶的能力，通过单细胞水平的荧光测定法来衡量。整合的标准是形成lac(+)重组菌落。结果发现，两种类型的供体显示出相同的基因转移频率，但在F(13)交配中整合的概率比在Hfr(1)交配中高10倍。在前者中，46%至97%的部分二倍体产生了重组菌落；在后者中，这一比例为2%至6%。
