Liu C, Rodina P
J Exp Med. 1966 Dec 1;124(6):1167-78. doi: 10.1084/jem.124.6.1167.
Tissue culture cells of human origin (HeLa, Lich, and AV(3)) were inoculated with the AL complex. By immunofluorescent staining, AL complex antigen was detectable in the cytoplasm of infected cells as punctate fluorescent granules during the early stage and as homogeneous fluorescence during the late stage of infection. By combining fluorescence and phase-contrast microscopy, many infected cells with cytoplasmic AL complex antigen were shown to have a normal nuclear morphology indistinguishable from uninfected cells. Initiation of AL complex infection was interpreted as occurring by transfer of transmissible factor(s) from cell to cell by contact and mutiplication of such factor(s) therein. Chick embryos were susceptible to AL complex infection following allantoic or amniotic inoculations. Antigen and infectious AL complex were demonstrable in the liver, brain, intestines, lungs, and embryonic membranes. Further investigations on AL complex and its relation to human disease are suggested.
将人类来源的组织培养细胞（HeLa、Lich和AV(3)）接种AL复合物。通过免疫荧光染色，在感染早期，感染细胞的细胞质中可检测到AL复合物抗原，呈点状荧光颗粒，而在感染后期呈均匀荧光。通过荧光显微镜和相差显微镜相结合，许多含有细胞质AL复合物抗原的感染细胞显示出与未感染细胞无法区分的正常核形态。AL复合物感染的起始被解释为通过接触在细胞间传递可传播因子并在其中增殖。鸡胚在尿囊或羊膜接种后易受AL复合物感染。在肝脏、大脑、肠道、肺和胚胎膜中可证实有抗原和传染性AL复合物。建议对AL复合物及其与人类疾病的关系进行进一步研究。
