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氧气对冻干粘质沙雷氏菌破坏作用的动力学研究。

Kinetic studies on the destructive action of oxygen on lyophilized Serratia marcescens.

作者信息

Dewald R R

出版信息

Appl Microbiol. 1966 Jul;14(4):568-72. doi: 10.1128/am.14.4.568-572.1966.

DOI:10.1128/am.14.4.568-572.1966
PMID:5333602
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC546783/
Abstract

Dried Serratia marcescens (ATTC strain 14041) cells were exposed to various partial pressures of oxygen and nitrogen. The colony-forming ability of the organisms was rapidly destroyed during exposure to oxygen but was unimpaired by exposure to purified nitrogen. The degree of inactivation depended upon temperature, time, and the partial pressure of oxygen, regardless of whether pure oxygen or dry air was used. The inactivation by oxygen followed the expression -1nN/N(0) = kO(2)t(1/2), where N(0) and N are the number of viable organisms before and after exposure respectively, [O(2)] is oxygen concentration, t is time, and k is the rate constant. At 25 C, k was 276 +/- 36 moles(-1/3) cc(1/2) hr(-1/3) for oxygen pressures between 5.5 and 258 torr. In the temperature range between -78 and 40 C, the rate constant may be expressed as k = 10(5.95+/-04.2) exp[(-430 +/- 26) cal/RT] moles(-1/3) cc(1/3) hr(-1/2).

摘要

将干燥的粘质沙雷氏菌(ATTC菌株14041)细胞暴露于不同的氧气和氮气分压下。在暴露于氧气期间,这些微生物的集落形成能力迅速被破坏,但暴露于纯氮气中则不受影响。失活程度取决于温度、时间和氧气分压,无论使用的是纯氧还是干燥空气。氧气导致的失活符合表达式-1nN/N(0) = kO(2)t(1/2),其中N(0)和N分别是暴露前后存活微生物的数量,[O(2)]是氧气浓度,t是时间,k是速率常数。在25℃时,对于5.5至258托之间的氧气压力,k为276 +/- 36摩尔(-1/3) 立方厘米(1/2) 小时(-1/3)。在-78至40℃的温度范围内,速率常数可表示为k = 10(5.95+/-04.2) exp[(-430 +/- 26) 卡/RT] 摩尔(-1/3) 立方厘米(1/3) 小时(-1/2) 。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb23/546783/a7d921ec3f38/applmicro00230-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb23/546783/a7d921ec3f38/applmicro00230-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb23/546783/a7d921ec3f38/applmicro00230-0094-a.jpg

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引用本文的文献

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本文引用的文献

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Factors Affecting the Viability of Serratia marcescens During Dehydration and Storage.脱水和储存过程中影响粘质沙雷氏菌生存能力的因素
J Bacteriol. 1946 Nov;52(5):565-73. doi: 10.1128/jb.52.5.565-573.1946.
2
Preservation of Microorganisms by Freeze-drying: II. The Destructive Action of Oxygen. Additional Stabilizers for Serratia marcescens. Experiments with Other Microorganisms.通过冷冻干燥保存微生物:II. 氧气的破坏作用。粘质沙雷氏菌的其他稳定剂。对其他微生物的实验
Appl Microbiol. 1961 May;9(3):256-62. doi: 10.1128/am.9.3.256-262.1961.
3
Electronspin resonance signals from lyophilized bacterial cells exposed to oxygen.
Appl Microbiol. 1968 Jul;16(7):1081-5. doi: 10.1128/am.16.7.1081-1085.1968.
来自暴露于氧气的冻干细菌细胞的电子自旋共振信号。
Nature. 1961 Oct 7;192:34-6. doi: 10.1038/192034a0.
4
QUANTITATIVE ASPECTS OF THE PROTECTION OF FREEZE-DRIED ESCHERICHIA COLI AGAINST THE TOXIC EFFECT OF OXYGEN.冻干大肠杆菌抗氧毒性作用的定量研究
J Gen Microbiol. 1963 Sep;32:321-9. doi: 10.1099/00221287-32-3-321.
5
FREE RADICAL FORMATION AND SURVIVAL OF LYOPHILIZED MICROORGANISMS.冻干微生物的自由基形成与存活
J Bacteriol. 1963 May;85(5):961-6. doi: 10.1128/jb.85.5.961-966.1963.
6
Factors affecting the viability of air-borne bacteria. II. The effect of chemical additives on the behavior of air-borne cells.影响空气传播细菌生存能力的因素。II. 化学添加剂对空气传播细胞行为的影响。
Can J Microbiol. 1960 Feb;6:71-87. doi: 10.1139/m60-010.
7
The effect of oxygen on freeze-dried Escherichia coli.氧气对冻干大肠杆菌的影响。
J Gen Microbiol. 1961 Feb;24:191-200. doi: 10.1099/00221287-24-2-191.
8
Substances which protect lyophilized Escherichia coli against the lethal effect of oxygen.
J Gen Microbiol. 1961 Jun;25:291-6. doi: 10.1099/00221287-25-2-291.
9
Preservation of Serratia marcescens by high-vacuum lyophilization.通过高真空冻干法保存粘质沙雷氏菌。
Appl Microbiol. 1966 Jul;14(4):561-7. doi: 10.1128/am.14.4.561-567.1966.
10
Effects of oxygen on aerosolized Serratia marcescens.氧气对雾化粘质沙雷氏菌的影响。
Appl Microbiol. 1965 Sep;13(5):781-7. doi: 10.1128/am.13.5.781-787.1965.