Ultrastructure of blebbing phenomenon and phagocytosis of blebs in laryngeal carcinoma.

The epithelial-connective tissue junction of invasive squamous cell laryngeal carcinoma was examined electron microscopically. The pleomorphic cytoplasmic protrusions known as blebs were observed on the lateral and basal surfaces of malignant keratinocytes. These blebs were pinched off from the malignant epithelial cells and were then observed in the connective tissue or in the intercellular spaces. After the pinching-off process some blebs were seen to be closely surrounded by pseudopods of phagocytizing histiocytic cells in the lamina propria or by adjacent malignant keratinocytes in the intercellular spaces. Since blebs are believed to be intact parts of viable cells this engulfment can be interpreted as cytophagocytosis. During cytophagocytosis the zeiotic blebs exhibited varying degrees of enzymatic digestion. The process of blebbing was discussed in respect to cellular locomotion of malignant keratinocytes during tumor invasion. This phenomenon seems to occur only in the preliminary stages of malignant tumor growth in the larynx.