Lynt R K, Solomon H M, Kautter D A, Lilly T
J Bacteriol. 1967 Jan;93(1):27-35. doi: 10.1128/jb.93.1.27-35.1967.
Clostridium botulinum type E antigens prepared from washed cells by either Formalin treatment or heating at 100 C were used for immunizing rabbits. Agglutination tests showed that high levels of antibody were produced by both types of preparations. Flagellar antigens were highly strain-specific, whereas the somatic antigens were sufficiently similar to produce complete cross-agglutination. One toxigenic strain produced toxigenic and nontoxigenic progeny which were physiologically and antigenically identical in all other respects. Other nontoxigenic strains whose growth, physiological, and morphological characters were identical to type E and strains which had some physiological differences completely cross-agglutinated with type E strains via the somatic antigen. Neither type of antiserum agglutinated other clostridia against which they were tested except for C. acetobutylicum. This reaction seems to be due to a nonspecific anamnestic response and does not appear to be related to the immunizing strains. The nontoxigenic strains studied seem to have no greater antigenic differences from type E strains than the type E strains have from each other.
由经福尔马林处理或在100℃加热的洗涤细胞制备的E型肉毒梭菌抗原用于免疫家兔。凝集试验表明，两种制剂均产生了高水平的抗体。鞭毛抗原具有高度的菌株特异性，而菌体抗原足够相似，可产生完全交叉凝集。一株产毒株产生了产毒和不产毒的后代，它们在所有其他方面在生理和抗原性上是相同的。其他生长、生理和形态特征与E型相同的不产毒株以及具有一些生理差异的菌株通过菌体抗原与E型菌株完全交叉凝集。除丙酮丁醇梭菌外，两种抗血清均未凝集它们所检测的其他梭菌。这种反应似乎是由于非特异性回忆反应，似乎与免疫菌株无关。所研究的不产毒株与E型菌株之间的抗原差异似乎并不比E型菌株彼此之间的差异更大。
