Anastasio K L, Soucheck J A, Sugiyama H
J Bacteriol. 1971 Jul;107(1):143-9. doi: 10.1128/jb.107.1.143-149.1971.
The bacteriocin, boticin E, was produced by only a few strains of those clostridia which are nontoxigenic but otherwise identical to Clostridium botulinum type E. Boticin preparations from four different strains had identical spectra against indicator cultures. Experiments with bacterial lawns showed boticin to be sporostatic for all tested nonproteolytic C. botulinum (types B, E, and F) and nontoxigenic type E-related strains which included the producing strains as well as those different from type E in the fermentation of one to three carbohydrates. Boticin had no detectable effect on vegetative cells of boticinogenic strains but killed those of all other strains whose spores were sensitive. Cultures that were growing in an agar medium were more sensitive to the bacteriocin than those growing in broth. Vegetative cells of indicator strains adsorbed boticin, but cells of a boticin-resistant mutant did not. Boticin did not lyse suspensions of vegetative cells which had been killed previously by exposure to air but lysed actively growing protoplasts and L-forms of a strain whose normal vegetative cells are susceptible to lysis. Sporostasis resulted from inhibition of germination rather than of outgrowth. Proteolytic strains of C. botulinum (types A, B, and F) were resistant to boticin E.
细菌素博蒂辛E仅由少数几株梭菌产生，这些梭菌不产生毒素，但在其他方面与E型肉毒杆菌相同。来自四种不同菌株的博蒂辛制剂对指示培养物具有相同的谱图。用菌苔进行的实验表明，博蒂辛对所有测试的非蛋白水解性肉毒杆菌（B型、E型和F型）以及与E型相关的非产毒菌株具有抑制孢子形成的作用，这些菌株包括产生博蒂辛的菌株以及在一至三种碳水化合物发酵方面与E型不同的菌株。博蒂辛对产生博蒂辛的菌株的营养细胞没有可检测到的影响，但能杀死所有其他孢子敏感菌株的营养细胞。在琼脂培养基中生长的培养物比在肉汤中生长的培养物对细菌素更敏感。指示菌株的营养细胞吸附博蒂辛，但博蒂辛抗性突变体的细胞不吸附。博蒂辛不会裂解先前因暴露于空气中而死亡的营养细胞悬液，但会裂解正常营养细胞易受裂解的菌株的活跃生长的原生质体和L型菌。孢子形成抑制是由发芽抑制而非生长抑制引起的。A、B和F型肉毒杆菌的蛋白水解菌株对博蒂辛E具有抗性。
