3-脱氧-2-氧代辛酸酯与来自大肠杆菌粗糙菌株的脂多糖的血清学及物理性质的关系。
The relation of 3-deoxy-2-oxo-octonate to the serological and physical properties of a lipopolysaccharide from a rough strain of Escherichia coli.
作者信息
Knox K W
出版信息
Biochem J. 1966 Jul;100(1):73-8. doi: 10.1042/bj1000073.
Abstract
- A water-soluble preparation of lipopolysaccharide was isolated from the extracellular lipopolysaccharide-phospholipid-protein complex formed by Escherichia coli A.T.C.C. 12408. 2. Heating at 100 degrees and pH4.6 caused a rapid decrease in serological activity concomitant with the release of 3-deoxy-2-oxo-octonate; aggregation of the molecules also occurred. 3. Further evidence that the release of 3-deoxy-2-oxo-octonate is related to loss of serological activity was obtained by comparing the effects of heating for 1hr. at 100 degrees and pH values between 5.0 and 9.0; detectable changes still occurred at pH7.5. 4. 3-Deoxy-2-oxo-octonate was isolated from the products of hydrolysis of lipopolysaccharide and shown to be an effective inhibitor of the precipitin reaction between lipopolysaccharide and homologous antiserum. 5. The possibility that 3-deoxy-2-oxo-octonate is joined to the lipopolysaccharide through a phosphodiester linkage is discussed.
摘要
- 从大肠杆菌A.T.C.C. 12408形成的细胞外脂多糖 - 磷脂 - 蛋白质复合物中分离出一种脂多糖的水溶性制剂。2. 在100℃和pH4.6条件下加热会导致血清学活性迅速下降，同时伴有3 - 脱氧 - 2 - 氧代辛酸的释放；分子也会发生聚集。3. 通过比较在100℃和pH值在5.0至9.0之间加热1小时的效果，获得了进一步的证据，证明3 - 脱氧 - 2 - 氧代辛酸的释放与血清学活性丧失有关；在pH7.5时仍可检测到变化。4. 从脂多糖水解产物中分离出3 - 脱氧 - 2 - 氧代辛酸，并证明它是脂多糖与同源抗血清之间沉淀素反应的有效抑制剂。5. 讨论了3 - 脱氧 - 2 - 氧代辛酸通过磷酸二酯键与脂多糖相连的可能性。
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