高离子强度对细菌核糖核酸生物活性成分沉降特性的影响。
The effect of high ionic strength on the sedimentation properties of the biologically active component of bacterial ribonucleic acid.
作者信息
Guérin M F, Hayes D H, Soffer R L
出版信息
Biochem J. 1966 Oct;101(1):256-64. doi: 10.1042/bj1010256.
Abstract
- The sedimentation properties of the fraction of bacterial RNA which stimulates the incorporation of amino acids into acid-insoluble material in vitro depend on the ionic strength of the sedimentation medium. 2. The different distributions of stimulatory activity found in centrifuged linear sucrose gradients loaded with bacterial RNA and containing 0.1m- or 0.6m-sodium chloride resemble closely the different sedimentation profiles of rapidly labelled RNA observed under the same conditions. 3. These findings agree with those of previous work of a similar nature (Willson & Gros, 1964) and demonstrate that the component of bacterial RNA preparations which stimulates the incorporation of amino acids into acid-insoluble peptides in vitro is contained in their rapidly labelled fraction.
摘要
- 细菌RNA中能在体外刺激氨基酸掺入酸不溶性物质的那部分的沉降特性取决于沉降介质的离子强度。2. 在含有0.1m或0.6m氯化钠的线性蔗糖梯度中加载细菌RNA后进行离心，发现刺激活性的不同分布与在相同条件下观察到的快速标记RNA的不同沉降图谱非常相似。3. 这些发现与之前类似性质的工作结果（威尔森和格罗斯，1964年）一致，并表明细菌RNA制剂中能在体外刺激氨基酸掺入酸不溶性肽的成分包含在其快速标记部分中。
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